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Handbook of Coherent-Domain Optical Methods pp 1157–1171Cite as

Confocal Laser Scanning Microscopy Using Scattering as the Contrast Mechanism

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Abstract

Confocal laser scanning microscopy (CLSM) is best known and widely used for fluorescence imaging. However, any commercial CLSM can be operated in reflectance mode by setting the microscope’s detector to accept the excitation laser wavelength. In this mode, the images are based on the scattering properties of the cell or tissue. This chapter discusses reflectance-mode CLSM (rCLSM), the mechanisms of contrast involved in such images, and examples of how rCLSM is being used to study tissue properties.

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Author information

Authors and Affiliations

  1. Oregon Health & Science University, 3303 SW Bond Ave, Portland, OR, 97239, USA

    Steven L. Jacques

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  1. Steven L. Jacques
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Correspondence to Steven L. Jacques .

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Editors and Affiliations

  1. , Chair of Optics and Biophotonics, Saratov State University, Astrakhanskaya str. 83, Saratov, 410012, Russia

    Valery V. Tuchin

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Jacques, S.L. (2013). Confocal Laser Scanning Microscopy Using Scattering as the Contrast Mechanism. In: Tuchin, V. (eds) Handbook of Coherent-Domain Optical Methods. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5176-1_28

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