Abstract
In a screen to identify zebrafish eye mutants, we isolated the good effort (gef) mutant. The retina of gef embryos is characterized by the successful initiation of the optic primordium and normal retinal development over the first 2 days post fertilization (dpf). The mutant retina, however, fails to continue to grow. Embryos from gef heterozygous incrosses were analyzed for cell death by acridine orange and by TUNEL labeling at 2 dpf. Significantly more TUNEL-positive and acridine orange-labeled dying cells were found in gef mutant embryos at 2 dpf relative to wild-type embryos. Because this time was earlier than any observable gross morphological differences, this cell death was likely the cause of the gross morphological defects. Meiotic mapping localized the mutation interval to a one-megabase interval on zebrafish chromosome 9.
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References
Vihtelic TS, Hyde DR (2002) Zebrafish mutagenesis yields eye morphological mutants with retinal and lens defects. Vision Res 42(4):535–540
Westerfield M (1993) The Zebrafish Book, 3rd edn. Univeristy of Oregon Press, Eugene
Lawson ND, Weinstein BM (2002) In vivo imaging of embryonic vascular development using transgenic zebrafish. Dev Biol 248(2):307–318
Gregg RG et al (2003) Positional cloning of the young mutation identifies an essential role for the Brahma chromatin remodeling complex in mediating retinal cell differentiation. Proc Natl Acad Sci U S A 100(11):6535–6540
Begemann G et al (2001) The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain. Development 128(16):3081–3094
Fadool JM et al (1997) Mutations affecting eye morphology in the developing zebrafish (Danio rerio). Dev Genet 20(3):288–295
Gross JM et al (2005) Identification of zebrafish insertional mutants with defects in visual system development and function. Genetics 170(1):245–261
Malicki J et al (1996) Mutations affecting development of the zebrafish retina. Development 123:263–273
Furutani-Seiki M et al (1996) Neural degeneration mutants in the zebrafish, Danio rerio. Development 123:229–239
Rodriguez M, Driever W (1997) Mutations resulting in transient and localized degeneration in the developing zebrafish brain. Biochem Cell Biol 75(5):579–600
Stainier DY et al (1996) Mutations affecting the formation and function of the cardiovascular system in the zebrafish embryo. Development 123:285–292
Fischer S et al (2007) Mutation of zebrafish caf-1b results in S phase arrest, defective differentiation, and p53-mediated apoptosis during organogenesis. Cell Cycle 6(23):2962–2969
Kaufman PD et al (1995) The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication. Cell 81(7):1105–1114
Plaster N et al (2006) p53 deficiency rescues apoptosis and differentiation of multiple cell types in zebrafish flathead mutants deficient for zygotic DNA polymerase delta1. Cell Death Differ 13(2):223–235
Yamaguchi M et al (2008) Mutation of DNA primase causes extensive apoptosis of retinal neurons through the activation of DNA damage checkpoint and tumor suppressor p53. Development 135(7):1247–1257
Daly FJ, Sandell JH (2000) Inherited retinal degeneration and apoptosis in mutant zebrafish. Anat Rec 258(2):145–155
Grunwald DJ et al (1988) A neural degeneration mutation that spares primary neurons in the zebrafish. Dev Biol 126(1):115–128
Nabatiyan A, Krude T (2004) Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of chromatin assembly during DNA synthesis. Mol Cell Biol 24(7):2853–2862
Acknowledgment
We thank Ronald R. Gregg (University of Louisville) for SNP mapping. This study was supported by the NIH (R01-EY018417) and the Center for Zebrafish Research at the University of Notre Dame.
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Bailey, T., Hyde, D. (2014). The Phenotype of the Good Effort Mutant Zebrafish is Retinal Degeneration by Cell Death and is Linked to the Chromosome Assembly Factor 1b Gene. In: Ash, J., Grimm, C., Hollyfield, J., Anderson, R., LaVail, M., Bowes Rickman, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 801. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3209-8_68
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DOI: https://doi.org/10.1007/978-1-4614-3209-8_68
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