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Basic Theories and Clinical Applications of Molecular Flow Cytometry

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Modern Clinical Molecular Techniques

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Abstract

Flow cytometry is a powerful tool for analyzing multiple parameters of individual cells and facilitating the identification of different cell types within heterogeneous populations. This technique has already become a routine method for diagnosis, prognosis, and monitoring of patients with many types of disorders and a crucial tool for accurately analyzing frequencies and relative values of cellular antigen expression. However, there are many technical obstacles involved in flow cytometric testing. This chapter provides an optimized immunostaining procedure with procedural variation for specific situations and a troubleshooting checklist.

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Author information

Authors and Affiliations

  1. ACM Medical Laboratory of Unity Health System, 160 Elmgrove Park, Rochester, NY, 14624, USA

    Hua-Kang Wu M.D., Ph.D., QCYM, MB(ASCP)CM, NYSCOQ

  2. Department of Medicine, Infectious Disease Division, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA

    Hongli Chen M.D., Ph.D.

  3. Molecular Genetic Technology Program, School of Health Professions, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Peter C. Hu Ph.D., MLS(ASCP)CMCGCM, MBCM

Authors
  1. Hua-Kang Wu M.D., Ph.D., QCYM, MB(ASCP)CM, NYSCOQ
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  2. Hongli Chen M.D., Ph.D.
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  3. Peter C. Hu Ph.D., MLS(ASCP)CMCGCM, MBCM
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Corresponding author

Correspondence to Hua-Kang Wu M.D., Ph.D., QCYM, MB(ASCP)CM, NYSCOQ .

Editor information

Editors and Affiliations

  1. , Molecular Genetic Technology Program, M.D. Anderson Cancer Center, 1515 Holcombe Blvd. Unit 2, Houston, 77030, Texas, USA

    Peter Hu

  2. , Department of Human Genetics, Emory University School of Medicine, 615 Michael St. NE, Suite 315, Atlanta, 30322, Georgia, USA

    Madhuri Hegde

  3. , Molecular Genetic Technology Program, M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Unit 2, Houston, 77030, Texas, USA

    Patrick Alan Lennon

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Wu, HK., Chen, H., Hu, P.C. (2012). Basic Theories and Clinical Applications of Molecular Flow Cytometry. In: Hu, P., Hegde, M., Lennon, P. (eds) Modern Clinical Molecular Techniques. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2170-2_22

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  • DOI: https://doi.org/10.1007/978-1-4614-2170-2_22

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-2169-6

  • Online ISBN: 978-1-4614-2170-2

  • eBook Packages: MedicineMedicine (R0)

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