Abstract
We developed a novel culture medium for primo microcells (PM) (sanals) by using a combination of fertilized egg albumen and RPMI-1640 medium (Gibco-Invitrogen, Carlsbad, CA, USA) (1:1). We observed the budding of a PM in real time at 21 min after incubation. At 52 min after incubation the bud of the PM grew to another PM-like structure connected with the first PM. Furthermore, we observed that the bud contained DNA by using the fluorescence imaging with acridine orange, which suggested elongation or growth of DNA from the first PM, named “a mother-PM,” in the budding process. However, we were able to observe only one case, so further study to cultivate PM with the same media or modified ones is needed.
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Acknowledgments
This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government, Ministry of Education, Science, and Technology (MEST) in 2010 (No. 2010–0025289) and by a Korean Pharmacopuncture Foundation grant funded by the Korean Pharmacopuncture Institute (KPI-2010-003).
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Lee, BC., Kang, DI., Soh, KS. (2012). Budding Primo Microcells (Sanals) in a Culture Medium with Fertilized Egg Albumen and RPMI Medium. In: Soh, KS., Kang, K., Harrison, D. (eds) The Primo Vascular System. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0601-3_24
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DOI: https://doi.org/10.1007/978-1-4614-0601-3_24
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