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Measurement of Vitamin D and its Metabolites by Gas Chromatography-Mass Spectrometry

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Vitamin D

Abstract

Gas-liquid chromatography (GC) has been very successfully applied to the analysis of a number of different groups of biologically important compounds. In particular, the analysis of the multiplicity of metabolites of secreted steroid hormones found in human urine has been greatly simplified by the introduction of GC (1). Using conventional packed columns (2) and wall-coated capillary columns (3), ‘profiles’ of varying degrees of complexity have been produced. Plasma steroids have also been analysed by GC using electron capture detection (4) and more recently using gas chromatography-mass spectrometry (GC-MS, 5).

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Authors
  1. Hugh L. J. Makin
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  2. David J. H. Trafford
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Editor information

Editors and Affiliations

  1. Department of Medicine, Mayo Clinic and Foundation, 200 First Street, S.W., Rochester, MN, 55905, USA

    Rajiv Kumar

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© 1984 Martinus Nijhoff Publishing, Boston/The Hague/Dordrecht/Lancaster

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Makin, H.L.J., Trafford, D.J.H. (1984). Measurement of Vitamin D and its Metabolites by Gas Chromatography-Mass Spectrometry. In: Kumar, R. (eds) Vitamin D. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2839-1_20

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  • DOI: https://doi.org/10.1007/978-1-4613-2839-1_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9793-2

  • Online ISBN: 978-1-4613-2839-1

  • eBook Packages: Springer Book Archive

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