Abstract
This outline will:
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1.
discuss the reasons for avoiding the covalent labeling of second antibodies in immunocytochemistry.
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2.
present a comparison between PAP and ABC methods.
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3.
discuss advantages and precautions in production and use of monoclonal antibodies for immunocytochemistry.
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4.
discuss advantages of using mouse or rat ClonoPAP made from monoclonal antiperoxidase.
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5.
show that immunocytochemistry with monoclonal antibodies permits dissection of biochemical processes in situ.
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6.
propose that the lesion in Alzheimer’s disease and related disorders involves a disturbance in a specific neurofilament phosphorylation site.
This research was supported by grants from the Multiple Sclerosis Society, The National Science Foundation (BNS 8205643), and The National Institutes of Health (NS 17665, NS 21681 (Javits Award) and HD 12921). The capable assistance of Francis Murant and Kristina Klingbiel is gratefully acknowledged.
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References
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Sternberger, L.A., Sternberger, N.H. (1985). Principles of Immunocytochemistry. In: Russo, J. (eds) Immunocytochemistry in Tumor Diagnosis. Developments in Oncology, vol 34. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2615-1_2
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