Abstract
Maize has a large haploid genome of about 3 × 106 kb organized on 10 chromosomes and resolved into 2,000 cM. The average physical distance to genetic distance is approximately 1,500 kb/cM. Large DNA molecules (up to 10 megabases—Mb) can be resolved with pulsed field gel electrophoresis (PFGE). This technique, together with yeast artificial chromosome vectors (YACs) and RFLP mapping, opens the prospect of isolating genes on the basis of their known genetic location. Protocols for high-molecular-weight (HMW) DNA isolation and resolution have been published for several plant species: Guzman and Ecker (1988) for Arabidopsis; Ganal and Tanksley (1989) for tomato; Cheung and Gale (1990) for wheat, barley, and rye; Sobral et al. (1990) for rice. A protocol for maize HMW DNA is described here and in the previous chapter.
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References
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© 1994 Springer-Verlag New York, Inc.
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Levy, A.A. (1994). Preparation of High-Molecular-Weight Maize DNA and Analysis by Pulsed-Field Gel Electrophoresis. In: Freeling, M., Walbot, V. (eds) The Maize Handbook. Springer Lab Manuals. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2694-9_87
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DOI: https://doi.org/10.1007/978-1-4612-2694-9_87
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-94735-8
Online ISBN: 978-1-4612-2694-9
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