Abstract
The polymerase chain reaction serves to amplify specifically the DNA sequence located between two primers selected by the investigator. This enables the investigator to, among other things, directly clone new alleles of previously cloned genes without constructing and screening a library. For an overview of the theory and application of PCR techniques we direct the reader to PCR Protocols (Innes et al. 1990) and a recent review (Bej et al. 1991). This chapter is devoted to techniques designed to overcome the difficulties that are commonly encountered in the application of PCR to maize DNA.
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© 1994 Springer-Verlag New York, Inc.
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Britt, A.B., Earp, D.J. (1994). The Polymerase Chain Reaction: Applications to Maize Transposable Elements. In: Freeling, M., Walbot, V. (eds) The Maize Handbook. Springer Lab Manuals. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2694-9_103
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DOI: https://doi.org/10.1007/978-1-4612-2694-9_103
Publisher Name: Springer, New York, NY
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