Abstract
Polymerase chain reaction (PCR) technology provides the most sensitive methods for detecting nucleic acids. These methods have proven exceedingly useful in detection of infectious agents in experimental and clinical settings (Kwok et al., 1987) and in analyses of unusual and precious small samples of tissue, such as those from extinct animals (Pääbo, 1989). They also have considerable potential for forensic applications (von Beroldingen et al., 1989). Most initial efforts to utilize the sensitivity of PCR were geared to issues of detection. For example, Saiki et al. (1988) showed that they could detect single target sequences (a β-globin gene) in 105 to 106 cells. Similarly, procedures for amplification of DNA from single cells exist (Li et al., 1988). However, these procedures were not designed to quantify amounts of target sequence in different samples.
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© 1994 Springer Science+Business Media New York
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Coen, D.M. (1994). Quantification of DNAs by the Polymerase Chain Reaction Using an Internal Control. In: Mullis, K.B., Ferré, F., Gibbs, R.A. (eds) The Polymerase Chain Reaction. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-0257-8_7
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DOI: https://doi.org/10.1007/978-1-4612-0257-8_7
Publisher Name: Birkhäuser, Boston, MA
Print ISBN: 978-0-8176-3750-7
Online ISBN: 978-1-4612-0257-8
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