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Fungi Allergens Produced by Solid-State Fermentation Process

Optimization and Allergen Characterization

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Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

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Abstract

Allergenic extracts were produced from Drechslera (Helminthosporium) monoceras biomass cultured by solid-state fermentation using wheat bran as the substrate. The main fermentation variables were selected by statistical design, and the optimized biomass yield (1.43 mg/[g of dry substrate · d]) was obtained at pH 9.5 and 45.8% moisture. The allergenic extracts were produced from crude extract by protein precipitation and polyphenol removal. Proteins in the range of 16–160 kDa were identified in the extracts. Their reactions in patients were characterized by in vivo cutaneous tests (positive in 40% of the atopic patients) and by dot-blotting assays.

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Author information

Authors and Affiliations

  1. School of Chemical Engineering, State University of Campinas, PO Box 6066, 13083-970, Campinas-SP, Brazil

    Salah D. M. Hasan & Maria H. A. Santana

  2. Laboratory of Mycology, Biomedical Science Institute, University of São Paulo, 05508-900, São Paulo, Brazil

    Walderez Gambale

  3. School of Medical Sciences, State University of Campinas, PO Box 6111, 13083-970, Campinas-SP, Brazil

    Ricardo L. Zollner

Authors
  1. Salah D. M. Hasan
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  2. Walderez Gambale
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  3. Ricardo L. Zollner
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  4. Maria H. A. Santana
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Corresponding author

Correspondence to Maria H. A. Santana .

Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, Oak Ridge, TN, USA

    Brian H. Davison (Conference Chair) & James W. Lee (Conference Chair) & 

  2. National Renewable Energy Laboratory, Golden, CO, USA

    Mark Finkelstein (Conference Co-Chair) & James D. McMillan (Conference Co-Chair) & 

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© 2003 Springer Science+Business Media New York

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Hasan, S.D.M., Gambale, W., Zollner, R.L., Santana, M.H.A. (2003). Fungi Allergens Produced by Solid-State Fermentation Process. In: Davison, B.H., Lee, J.W., Finkelstein, M., McMillan, J.D. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0057-4_33

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  • DOI: https://doi.org/10.1007/978-1-4612-0057-4_33

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6592-4

  • Online ISBN: 978-1-4612-0057-4

  • eBook Packages: Springer Book Archive

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