How to Conduct Cell Culture

Abstract

Cell culture is an invaluable tool for the experimental researcher. It allows for greater variable control and faster hypothesis testing, and it is cheaper than animal models. A thorough understanding of both the theory behind cell culture experiments and the techniques involved is paramount for a successful research career. In this chapter we cover the basics of cell culture and give you the tools to start working with cells. We begin by describing the technical aspects of cell culture and the importance of establishing good cell culture practices. Following this simple advice will make a huge difference in your experimental success rate. We then discuss theoretical aspects for relevant experimental design, including considerations about selecting the appropriate cell type and environment, and how this selection process directly affects your results. Carefully designed cell experiments will save you time and money and give you a better understanding of the problem you are trying to address.