Abstract
The aim of this chapter is to describe the requirements for combining the patch-clamp technique with the fluorometric monitoring of changes in ion concentration in single cells in brain slice preparations (Llano et al., 1991; Kano et al., 1992; Konnerth et al., 1992; Schneggenburger et al., 1993a,b). Additional details of the patch-clamp technique in brain slices and of the different procedures for fluorometric ion measurements are given in other chapters (Chapters 8 and 16) of this volume and elsewhere (Smith et al., 1983; Tsien and Poenie, 1986; Neher, 1989).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alford, S., Frenguelli, B. G., Schofield, J. G., and Collingridge, G. L., 1993, Characterization of Ca“- signals induced in hippocampal CAI neurones by the synaptic activation of NMDA receptors, J. Physiol. 469: 693–716.
Almers, W., and Neher, E., 1985, The Ca signal from fura-2 loaded mast cells depends strongly on the method of dye-loading, FEBS Lett. 192: 13–18.
Blanton, M. G., LoTurco, J., and Kriegstein, A. R., 1989, Whole cell recording from neurons in slices of reptilian and mammalian cerebral cortex, J. Neurosci. Methods 30: 203–210.
Bliss, T. V., and Collingridge, G. L., 1993, A synaptic model of memory: Long-term potentiation in the hippocampus, Nature 361: 31–39.
Callaway, E. M., and Katz, L. C., 1993, Photostimulation using caged glutamate reveals functional circuitry in living brain slices, Proc. Natl. Acad. Sci. USA 90: 7661–7665.
Connor, J. A., 1986, Digital imaging of free calcium changes and of spatial gradients in growing processes in single mammalian central nervous system cells, Proc. Natl. Acad. Sci. USA 83: 6179–6183.
Dreessen, J., 1992, Quantitative Messung der intrazellulären Ca++-Konzentration mit einem Bildverarbeitungssystem bei der Untersuchung der synaptischen Plastizität in den Purkinje-Zellen des Kleinhirns, Ph.D. Thesis, University of Hannover.
Edwards, E. A., Konnerth, A., Sakmann, B., and Takahashi, T., 1989, A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system, Pfltigers Arch. 414: 600–612.
Eilers, J., Augustine, G. J., and Konnerth, A., 1994, Calcium signals in fine dendrites and spines of cerebellar Purkinje neurons, Pfügers Arch. 426: R27.
Fine, A., Amos, W. B., Durbin, R. M., and McNaughton, P. A., 1988, Confocal microscopy: Applications in neurobiology, Trends Neurosci. 11: 346–351.
Gamble, E., and Koch, C., 1987, The dynamics of free calcium in dendritic spines in response to repetitive synaptic input, Science 236: 1311–1315.
Grynkiewicz, G., Poenie, M., and Tsien, R., 1985, A new generation of Ca’ indicators with greatly improved fluorescence properties, J. Biol. Chem. 260: 3440–3450.
Haugland, R. P., 1992, Handbook of Fluorescent Probes and Research Chemicals (K. Larison, ed.), Molecular Probes, Inc., Eugene, OR.
Kano, M., Rexhausen, U., Dreessen, J., and Konnerth, A., 1992, Synaptic excitation produces a long-lasting rebound potentiation of inhibitory synaptic signals in cerebellar Purkinje cells, Nature 356: 601–604.
Kao, J. P. Y., Harootunian, A. T., and Tsien, R. Y., 1989, Photochemically generated cytosolic calcium pulses and their detection by fluo-3, J. Biol. Chem. 264: 8179–8184.
Kaplan, J. H., and Somlyo, A. P., 1989, Flash photolysis of caged compounds: New tools for cellular physiology, Trends Neurosci. 12: 54–59.
Knöpfel, T., Vramesic, C., Staub, V. C., and Gähwiler, B., 1990, Climbing fiber responses in olivo-cerebellar slice cultures. II. Dynamics of cytosolic calcium in Purkinje cells, Eur. J. Neurosci. 3: 343–348.
Konnerth, A., 1990, Patch-clamping in slices of mammalian CNS, Trends Neurosci. 13: 321–323.
Konnerth, A., Dreessen, J., and Augustine, G. J., 1992, Brief dendritic calcium signals initiate long-lasting synaptic depression in cerebellar Purkinje cells, Proc. Natl. Acad. Sci. USA 89: 7051–7055.
Lasser-Ross, N., Miyakawa, H., Lev-Ram, V., Young, S. R., and Ross, W. N., 1991, High time resolution fluorescence imaging with a CCD camera, J. Neurosci. Methods 36: 253–261.
Lewis, R. S., and Cahalan, M. D., 1989, Mitogen-induced oscillations of cytosolic Ca“ and transmembrane Ca’ current in human leukemic T cells, Cell Regul. 1: 99–112.
Llano, I., Dreessen, J., Kano, M., and Konnerth, A., 1991, Intradendritic release of calcium induced by glutamate in cerebellar Purkinje cells, Neuron 7: 577–583.
Malenka, R. C., Lancaster, B., and Zucker, R. S., 1992, Temporal limits on the rise in postsynaptic calcium required for the induction of long-term potentiation, Neuron 9: 121–128.
Mathias, R. J., Cohen, I. S., and Oliva, C., 1990, Limitations of the whole cell patch clamp technique in the control of intracellular solutions, Biophys. J. 58: 759–770.
Miyakawa, H., Lev-Ram, V., Lasser-Ross, N., and Ross, W. N., 1992, Calcium transients evoked by climbing fiber and parallel fiber synaptic inputs in guinea pig cerebellar Purkinje neurons, J. Neurophysiol. 68: 1178–1189.
Müller, W., and Connor, J. A., 1991, Dendritic spines as individual neuronal compartements for synaptic Ca’-’ responses, Nature 354: 73–76.
Murphy, T. H., Baraban, J. M., Wier, W. G., and Blatter, L. A., 1994, Visualization of quantal synaptic transmission by dendritic calcium imaging, Science 263: 529–532.
Neher, E., 1988, The influence of intracellular calcium concentration on degranulation of dialysed mast cells from rat peritoneum, J. Physiol. 395: 193–214.
Neher, E., 1989, Combined fura-2 and patch clamp measurements in rat peritoneal mast cells, in: Neuromuscular Junction ( L. Sellin, R. Libelius, and S. Thesleff, eds.), pp. 65–76, Elsevier, Amsterdam.
Neher, E., and Augustine, G. J., 1992, Calcium gradients and buffers in bovine chromaffin cells, J. Physiol. 450: 273–301.
Pusch, M., and Neher, E., 1988, Rates of diffusional exchange between small cells and a measuring patch pipette, Pflügers Arch. 411: 204–211.
Regehr, W. G., and Tank, D. W., 1992, Calcium concentration dynamics produced by synaptic activation of CA 1 hippocampal pyramidal cells, J. Neurosci. 12: 4202–4223.
Rexhausen, U., 1992, Bestimmung der Diffusionseigenschaften von Fluoreszenzfarbstoffen in verzweigten Nervenzellen unter Verwendung eines rechnergesteuerten Bildverarbeitungssystems, Diploma Thesis, University of Göttingen.
Schneggenburger, R., Zhou, Z., Konnerth, A., and Neher, E., 1993a, Fractional contribution of calcium to the cation current through glutamate receptor channels, Neuron 11: 133–143.
Schneggenburger, R., Tempia, P., and Konnerth, A., 1993b, Glutamate-and AMPA-mediated calcium influx through glutamate receptor channels in medial septal neurons, Neuropharmacology 32: 1221–1228.
Smith, S. J., MacDermott, A. B., and Weight, F. F., 1983, Detection of intracellular Ca’ transients in sympathetic neurones using arsenazo III, Nature 304: 350–352.
Stuart, G. J., Dodt, H. U., and Sakmann, B., 1993, Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy, Pflügers Arch. 423: 511–518.
Tsien, R. Y., and Poenie, M., 1986, Fluorescence ratio imaging: A new window into intracellular ionic signalling, Trends Biochem. Sci. 11: 450–455.
Tsien, R. Y., and Waggoner, A., 1990, Fluophores for confocal microscopy: Photophysics and photochemistry, in: Handbook of Biological Confocal Microscopy ( J. B. Pawley, ed.), pp. 169–178, Plenum Press, New York.
Zhou, Z., and Neher, E., 1993, Calcium permeability of nicotinic acetylcholine receptor channels in bovine adrenal chromaffin cells, Pflügers Arch. 425: 511–517.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Eilers, J., Schneggenburger, R., Konnerth, A. (1995). Patch Clamp and Calcium Imaging in Brain Slices. In: Sakmann, B., Neher, E. (eds) Single-Channel Recording. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-1229-9_9
Download citation
DOI: https://doi.org/10.1007/978-1-4419-1229-9_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-1230-5
Online ISBN: 978-1-4419-1229-9
eBook Packages: Springer Book Archive