Mammalian microRNAs (miRNAs) are small RNAs of 18–25 nucleotides (nt) in length that are transcribed from the genome and serve a variety of regulatory functions. Recent studies have verified that miRNAs in addition to playing anti-pathogen roles in plants and lower eukaryotes also serve important roles in defending mammalian cells against endogenous and exogenous viral infection. In a setting where miRNAs may act to restrict viral infection, it stands to reason that viruses may have a vested interest to change the miRNA expression profile of infected cells. Here, we describe our experience in establishing a microarray for measuring 327 human miRNAs. We illustrate an example whereby this microarray approach was used to measure miRNA changes in human cells transfected to express human immunodeficiency virus proteins.
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Yeung, M.L., Jeang, KT. (2008). Microarray Profiling of microRNA Changes in Cells That Express HIV-1 Proteins. In: Ying, SY. (eds) Current Perspectives in microRNAs (miRNA). Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8533-8_21
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DOI: https://doi.org/10.1007/978-1-4020-8533-8_21
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