Abstract
In the cyanobacterium Synechocystis sp. PCC 6803 at least three DnaK proteins are functionally expressed, two of which (DnaK2 and DnaK3) are essential for cell viability. The Synechocystis DnaK2 protein shows the highest sequence similarity to Hsp70 proteins from other bacteria and chloroplasts. Therefore, this protein has been suggested to have a privileged function in vivo. We show here that the expression of dnaK2 alters most significantly when Synechocystis cells are grown under different cellular stresses. Furthermore, the expression of dnaK3 also varies at certain stress conditions.
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References
Fulda S, Mikkat S, Huang F, Huckauf J, Marin K, Norling B, Hagemann M (2006) Proteome analysis of salt stress response in the cyanobacterium Synechocystis sp. strain PCC 6803. Proteomics 6:2733-2745.
Kanesaki Y, Suzuki I, Allakhverdiev SI, Mikami K, Murata N (2002) Salt stress and hyperosmotic stress regulate the expression of different set of genes in Synechocystis sp. PCC 6803. Biochem Biophys Res Commun 290:339-348.
Mary I, Tu CJ, Grossman A, Vaulot D (2004) Effects of high light transcripts of stress-associated genes for the cyanobacteria Synechocystis sp. PCC 6803 and Prochlorococcus MED4 and MIT9313. Microbiology 150:1271-1281.
Nimura K, Takahashi H, Yoshikawa H (2001) Characterization of the dnaK multigene family in the cyanobacterium Synechococcus sp. strain PCC 7942. J Bacteriol 183:1320-1328.
Rippka R, Derulles J, Waterbury JB, Herdman M, Stanie RY (1979) Genetic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1-61.
Rupprecht E, Gathmann S, Fuhrmann E, Schneider D (2007) Three different DnaK proteins are functionally expressed in the cyanobacterium Synechocystis sp. PCC 6803. Microbiology 153:1828-1841.
Varvasovszki V, Glatz A, Shigapova N, Josvay K, Vigh L, Horvath I (2003) Only one dnaK homolog, dnaK2, is active transcriptionally and is essential in Synechocystis. Biochem Biophys Res Commun 305:641-648.
Suzuki I, Simon WJ, Slabas AR (2006) The heat shock response of Synechocystis sp. PCC 6803 analysed by transrciptomics and proteomics. J Exp Bot 57: 1573-1578.
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Rupprecht, E., Fuhrmann, E., Schneider, D. (2008). Stress Regulated DnaK Expression in Synechocystis sp. PCC 6803. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_286
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_286
Publisher Name: Springer, Dordrecht
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