Abstract
Efficient photosynthetic energy transduction and its regulation depends on a precise supramolecular arrangement of plant photosystem II (PSII) complex in grana membranes of chloroplasts. The topography of isolated PSII supercomplexes and their supramolecular organization in grana membrane preparations has been visualized by high resolution atomic force microscopy in air with tapping mode and an active feedback control to minimize tip-sample interactions. Taking the protruding water splitting apparatus as a topographic marker for PSII, its distribution and orientation in isolated grana membrane was analyzed. A new mathematical procedure was established which revealed a preference for a parallel alignment of PSII.
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Kirchhoff, H., Büchel, C., Chi, L., Lenhert, S., Nield, J. (2008). Investigating The Organization Of Photosystem Ii In Spinach Photosynthetic Membranes By Atomic Force Microscopy. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_174
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_174
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6707-5
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