Abstract
Arabidopsis Chlororespiratory Reduction 1 (Crr1) Mutants Were Isolated By Chlorophyll Fluorescence Imaging On The Basis Of Their Lack Of Chloroplast Nad(P)H Dehydrogenase (Ndh) Activity. Crr1 Is Homologous To Dihydrodipicolinate Reductase (Dhpr), Which Functions In A Lysine Biosynthesis Pathway. However, The Dhp-Binding Motif Was Not Conserved In Crr1, And The Crr1 Defect Specifically Caused The Destabilization Of Ndh, Implying That Crr1 Is Not Involved In Lysine Biosynthesis In Arabidopsis. Crr1 Contained A Chloroplast Targeting Signal And An Nad(P)H-Binding Motif. In Addition, Crr1 Was Expressed Only In Photosynthetic Tissue As Other Nuclear-Encoded Genes For Ndh Subunits. These Characteristics Indicated That Crr1 Was A Candidate For The Electron Donor-Binding Subunit Of Ndh. However, Crr1 Was Detected In The Stroma But Not In The Thylakoid Membranes, Where Ndh Is Localized. Furthermore, Crr1 Was Stable Even In Absence Of Ndh. These Results Suggest That Crr1 Is Involved In Biogenesis Or Stabilization Of Ndh, Possibly Via The Reduction Of An Unknown Substrate.abstract
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Shimizu, H., Shikanai, T. (2008). CRR1 Is Specifically Involved in Accumulation of the Chloroplast NDH Complex Despite Its Homology to DHPR. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_151
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_151
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