Abstract
The Manganese Stabilising Protein (MSP) is an extrinsic subunit of Photosystem II, which is essential for optimal levels of oxygen production during photosynthesis under physiological conditions. The MSP is present in all oxygenic phototrophs including Gloeobacter violaceus, which is the most primitive of contemporary cyanobacteria, having diverged very early from the common ancestor. Because G. violaceus is difficult to culture and is extremely slow growing, it has not yet been isolated biochemically. Instead we report here on our protocols for the expression and purification of rMSP in E. coli inclusion bodies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Berthold DA, Babcock GT, Yocum CF (1981) A highly resolved, oxygen-evolving photosystem-II preparation from spinach thylakoid membranes - Electron para-magnetic resonance and electron transport properties. FEBS Lett 134:231-234.
De Las Rivas J, Barber J (2004) Analysis of the structure of the PsbO protein and its implications. Photosynth Res 81:329-343.
Ferreira KN, Iverson TM, Maghlaoui K, Barber J, Iwata S (2004) Architecture of the photosynthetic oxygen-evolving center. Science 303:1831-1838.
Ishikita H, Saenger W, Loll B, Biesiadka J, Knapp EW (2006) Energetics of a possible proton exit pathway for water oxidation in photosystem II. Biochemistry 45:2063-2071.
Loll B, Kern J, Saenger W, Zouni A, Biesiadka J (2005) Towards complete cofactor arrangement in the 3.0 Ã… resolution structure of photosystem II. Nature 438:1040-1044.
Murray JWB (2007) Structural characteristics of chan-nels and pathways in photosystem II including the identification of an oxygen channel. J Struct Biol 159:228-237.
Nakamura Y, Kaneko T, Sato S, Mimuro M, Miyashita H, Tsuchiya T, Sasamoto S, Watanabe A, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Nakazaki N, Shimpo S, Takeuchi C, Yamada M, Tabata S (2003) Complete genome structure of Gloeobacter violaceus PCC 7421, a cyano-bacterium that lacks thylakoids. DNA Res 10:137-145.
Rippka RWJ, Cohnen-Bazire G (1974) A cyanobacterium which lacks thylakoids. Arch Microbiol 100:419-436.
Williamson AL, Hillier W, Wydrzynski T (2007) The importance of protein-protein interactions for optimis-ing oxygen activity in photosystem II: Reconstitution with a recombinant thioredoxin-manganese stabilising protein. Photosynth Res 92:305-314.
Wu JT, Masri N, Lee W, Frankel LK, Bricker TM (1999) Random mutagenesis in the large extrinsic loop E and transmembrane alpha-helix VI of the CP 47 protein of photosystem II. Plant Mol Biol 39:381-386.
Wydrzynski T, Hillier W, Messinger J (1996) On the functional significance of substrate accessibility in the photosynthetic water oxidation mechanism. Physiol Plantarum 96:342-350.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2008 Springer Science + Business Media, B.V.
About this paper
Cite this paper
Williamson, A., Hillier, W., Wydrzynski, T. (2008). Expression of the Manganese Stabilising Protein from a Primitive Cyanobacterium. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_119
Download citation
DOI: https://doi.org/10.1007/978-1-4020-6709-9_119
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6707-5
Online ISBN: 978-1-4020-6709-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)