Abstract
This investigation shows diurnal variations in the xylem-sap composition of poplar. All major macronutrients reached a maximum concentration in the first half of the light period and decreased to the middle of the night. The relative abundance of the nutrients did not change during the day. The sap flow which responded very fast to the environmental changes (2.2 fold increase within 10–20 min of illumination) reached a maximum value in the second half of the light period. Transpiration (and photosynthesis) was constant throughout the light phase. The calculated translocation rates displayed a maximum in the first half of the light period and, therefore, did not fit the time course of sap flow. During the night, translocation rates were 63–69% lower than the maximum. The regulation of nutrient translocation is discussed taking the active xylem loading into account. The axial distribution located the nitrate assimilation in younger and storage of nitrate (and other macro nutrients) in older leaves. However, the sap flow was greater in younger shoot sections compared to older sections. We assume that the greater demand for nitrate in the younger shoot section was satisfied via an increased volume flow rather an increased nitrate concentration. Salt treatment mainly affected young leaves increasing their Na+ and Cl− content on the expense of Mg2+ and NO3 − content respectively. A threshold (pH 3.25) was observed concerning the effect of low pH on in vivo sap flow.
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References
Crossett, R.N. (1968). Effect of light upon the translocation of phosphorus by seedlings of Hordeum vulgare (L.). Aust. J. Biol. Sci. 21, 225–233.
De Cires, A., De la Torre, A., Delgado, B. and Lara, C. (1993) Role of light and CO2 fixation in the control of nitrate reductase activity in barley leaves. Planta, 190, 227–283.
Delhon, P., Gojon, A., Tillard, P. and Passama, L. (1995a) Diurnal regulation of NO3 - uptake in soybean plants I. Changes in NO3 - influx, efflux, and N utilization in the plant during the day/night cycle. J. Exp. Bot., 46, 1585–1594.
Delhon, P., Gojon, A., Tillard, P. and Passama, L. (1995b) Diurnal regulation of NO3 - uptake in soybean plants II. Relationship with accumulation of NO3 - and asparagine in the roots. J. Exp. Bot., 46, 1595–1602.
Else, M.A., Davis, W.J., Whitford, P.N., Hall, K.C. and Jackson, M.B. (1994) Concentrations of abscisic acid and other solutes in xylem sap from root systems of tomato and castor-oil plants are distorted by wounding and variable sap flow rates. J. Exp. Bot., 45, 317–323.
Else, M.A., Hall, K.C., Arnold, G.M., Davis, W.J. and Jackson, M.B. (1995) Export of abscisic acid, 1-aminocyclo-propane-1carboxylic acid, phosphate, and nitrate from roots to shoots of flooded tomato plants. Accounting for effects of xylem sap flow rate on concentration and delivery. Plant Physiol., 107, 377–384.
Gaymard, F., Pilot, G., Lacombe, B., Bouchez, D., Bruneau, D., Boucherez, J., Michaux-Ferriere, N., Thibaud, J.B. and Sentenac, H. (1998) Identification and disruption of a plant shaker-like outward channel involved in K+ release into the xylem sap. Cell, 94, 647–655.
Gonzales-Real, M.M. and Baille, A. (2000) Changes in leaf photosynthetic parameterswith leaf position and nitrogen content within a rose plant canopy (Rosa hybrida). Plant Cell Environ., 23, 351–357.
Grignon, C. and Sentenac, H. (1991) pH and ionic conditions in the apoplast. Ann. Review Plant Physiol. Plant Mol. Biol., 42, 103–128.
Haruta, M. and Constable, C.P. (2003) Rapid alkalization factors in poplar cultures. Peptide isolation, cDNA cloning, and differential expression in leaves and methyl jasmonate treated cells. Plant Physiol., 131, 814–823.
Huber, J.L., Huber, S.C., Campell, W.H. and Redinbaugh, M.G. (1992a) Reversible light/dark modulation of spinach leaf nitrate reductase activity involves protein phosphorylation. Arch. Biochem. Biophys., 296, 58–65.
Huber, J.L., Huber, S.C., Campell, W.H. and Redinbaugh, M.G. (1992b) Apparent dependence of the light acitvation of nitrate reductase and sucrose-phosphate synthase activities in spinach leaves on protein synthesis. Plant Cell. Physiol., 33, 639–646.
Huber, S.C., Huber, J.L., Campell, W.H. and Redinbaugh, M.G. (1992c) Comparative studies of the light modulation of nitrate reductase and sucrose-phosphate synthase activities in spinach leaves. Plant Physiol., 100, 706–712.
Jeschke, W.D. and Pate, J.S. (1991) Modelling of water uptake, flow and utilization of C, N and H2O within whole plants of Ricinus communis L. Based on empirical data. J. Plant Physiol., 137, 488–498.
Kopriva, S., Muheim, R., Koprivova, A., Trachsel, N., Catalano, C., Suter, M. and Brunold, C. (1999) Light regulation of assimilatory sulphate reduction in Arabidopsis thaliana. Plant J., 20, 37–44.
Köhler, B., Wegner, L., Osipov, V. and Raschke, K. (2002) Loading of nitrate into the xylem: apoplastic nitrate controls the voltage dependence of X-QUAC, the main conductance in xylem-parenchyma cells of barley roots. Plant J., 30, 133–142.
Marschner, H. (1995) Mineral nutrition of higher plants. Second edition, Academic Press, London.
Marschner, H., Kirkby, E.A. and Engels, C. (1997) Importance of cycling and recycling of mineral nutrients within plants for growth and development. Bot. Acta, 110, 265–273.
Mattson, M., Lundborg, T. and Larsson, C. (1988) Nitrate utilization in barley: relations to nitrate supply and light/dark cycles. Physiol. Plant, 73, 380–386.
Muller, B. and Touraine, B. (1992) Inhibition of NO3 - uptake by various phloem-translocated amino acids in soybean seedlings. J. Exp. Bot., 43, 617–623.
Oji, Y., Otani, Y., Hosomi, Y., Wakiuchi, N. and Shiga, H. (1989). Nitrate reduction in in root and shoot and exchange of reduced nitrogen between organs in two-row barley seedlings under light-dark cycles. Planta. 179, 359–366.
Ourry, A., Macduff, J.H., Prudhomme, M.P. and Boucaud, J. (1996) Diurnal variation in the simultaneous uptake and ,sink’ allocation of NH4 + and NO3 - by Lolium perenne in flowing solution culture. J. Exp. Bot., 47, 1853–1863.
Peuke, A.D. and Jeschke, W.D. (1998) The effects of light on induction, time courses, and kinetic patterns of net nitrate uptake in barley. Plant Cell Environ., 21, 765–774.
Poirier, Y., Thoma, S., Sommerville, C. and Schiefelbein, J. (1991) A mutant of Arabidopsis deficient in xylem loading of phosphate. Plant Physiol., 97, 1087–1093.
Rideout, J.W., Chaillou, S., Raper, C.D. and Morot-Gaudry, J.F. (1994) Ammonium and nitrate uptake by soybean during recovery from nitrogen deprivation. J. Exp. Bot., 45, 23–33.
Rufty, T.W., Mackown, C.T. and Volk, R.J. (1989). Effects of altered carbohydrate availability on whole-plant assimilation of 15NO-3. Plant Physiol., 89, 457–463.
Santora, L.G. and Magalhaes, A.C.N. (1983) Changes in nitrate reductase activity during development of soybean leaf. J. Plant Physiol., 112, 113–121.
Scaife, A. and Schloemer, S. (1994). The diurnal pattern of nitrate uptake and reduction by spinach (Spinacia oleracea L.). Ann. Bot., 73, 337–343.
Schill, V., Hartung, W., Orthen, B. and Weiselseel, M.H. (1996) The xylem sap of maple (Acer platanoides L.) trees-sap obtained by a novel method shows changes with season and height. J. Exp. Bot., 47, 123–133.
Schurr, U. and Schulze, E.D. (1995) The concentration of xylem sap constituents in root exudate, and in sap from intact, transpiring castor bean plants (Ricinus communis L.). Plant Cell Environ., 18, 409–420.
Shaner, D.L. and Boyer, J.S. (1976) Nitrate reductase activity in maize (Zea mays L.) leaves. II Reguation by nitrate flux at low leaf water potential. Plant Physiol., 58, 505–509.
Siebrecht, S. and Tischner, R. (1999) Changes in the xylem exudate composition of poplar (Populus tremulaxP. alba) - dependent on the nitrogen and potassium supply. J. Exp. Bot., 50, 1797–1806.
Smith, J.A.C. (1991) Ion transport and the transpiration stream. Bot. Acta, 104, 416–421.
Tanner, W. and Beevers, H. (1990) Does transpiration have an essential function in long-distance ion transport in plants? Plant Cell Environ., 13, 745–750.
Touraine, B., Muller, B. and Grignon, C. (1992) Effect of phloem-translocated malate on NO3 - uptake by roots of intact soybean plants. Plant Physiol., 99, 1118–1123.
Wei, C., Tyree, M.T. and Steudle, E. (1999) Direct measurement of xylem pressure in leaves of intact maize plants. A test of the cohesion-tension theory taking hydraulic architecture into consideration. Plant Physiol., 121, 1191–1206.
Wegner, L.H. and Raschke, K. (1994) Ion channels in the xylem parenchyma of barley roots: a procedure to isolate protoplasts from this tissue and a patch-clamp exploration of salt passageways into xylem vessels. Plant Physiol., 105, 799–813.
Wolf, D., Jeschke, W.D. and Hartung, W. (1990) Concentrations and transport of solutes in xylem and phloem along the leaf axis of NaCl-treated Hordeum vulgare. J. Exp. Bot., 41, 1133–1141.
Zimmermann, U., Meinzer, F.C., Benkert, R., Zhu, J.J., Schneider, H., Goldstein, G., Kuchenbrod, E. and Haase, A. (1994) Xylem water transport: is the available evidence consistent with the cohesion theory? Plant Cell Environ., 17, 1169–1181.
Zweifel, R., Item, H. and Hasler, R. (2001) Link between dirunal stem radius changes and tree water relations. Tree Physiol., 12–13, 869–877.
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Siebrecht, S., Fiebelkorn, G., Tischner, R. (2007). Changes in Composition of the Xylem Sap as well as in Ion Fluxes in Populus Tremula X Alba L. Xylem In Dependence on Exogenous Factors. In: Sattelmacher, B., Horst, W.J. (eds) The Apoplast of Higher Plants: Compartment of Storage, Transport and Reactions. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5843-1_20
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DOI: https://doi.org/10.1007/978-1-4020-5843-1_20
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