Abstract
For the production of recombinant proteins for clinical use animal cell cultivation is used. Only these cells are able to perform correct folding and glycosylation of the desired protein. As the production process is expensive and long, cheaper and/or swifter cultivation routines are required. Chinese hamster ovary (CHO) cells were used to examine the expansion of cells and the production of recombinant human growth hormone in different cell culture systems which are supposed to achieve higher cell densities and product concentrations compared to conventional cell culture systems. The CHO cells were grown in suspension in serum-free, low-protein medium. Five different culture systems were used for batch-cultivation: Biostat B, BelloCell 500, spinner flask, RCCS-D and miniPERM. The systems differed in oxygen supply and medium agitation. While cells are agitated by stirrers in Biostat B and spinner flask, the whole medium is revolved in BelloCell 500, RCCS-D and miniPERM. Unlike the other systems the BelloCell 500 retains the CHO cells on a matrix. The aim was to maximize cell growth and productivity, which was achieved best in BelloCell and RCCS-D. In a second step the influence of temperature on growth and product formation was examined.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Birch, J.R., Froud S.J., 1994, Mammalian cell culture systems for recombinant protein production, Biologicals, 22: 127–133
Bloemkolk, J.W., Gra, M.R., Merchant, F., Mosmann, T.R., 1992, Effect of temperature on hybridoma cell cycle and mAb production, Biotechnol.Bioeng., 42: 427–431
Hauser, HJ., Wagner, R., 1997, Mammalian cell biotechnology in protein production, Walter de Gruyter Berlin New York, 3.1: 279
Hu, W.-S., Piret, J.M., 1992, Mammalian cell culture processes, Current Opinion in Biotechnology, 3: 110–114
MacDonald, C., 1990, Development of new cell lines for animal cell biotechnology, Critical reviews in Biotechnology, 2(10): 155–178
Ribela, M.-T. C.P., Gout, P.W., Bartolini, P., 2003, Synthesis and chromatographic purification of recombinant human pituitary hormones, Journal of Chromatography B, 790: 285–316
Rössler, B., Lübben, H., Kretzmer, G., 1996, Temperature: a simple parameter for process optimization in fed-batch cultures of recombinant Chinese hamster ovary cells, Enzyme and Microbial Technology, 18: 423–427
Simmonsen, C.C, McGrogan, M., 1994, The molecular biology of production cell lines, Biologicals 22: 85–94
Sureshkumar, G.K., Mutharasan, R., 1991, The Influence of temperature on a mouse-mouse hybridoma growth and momoclonal antibody production, Biotechnol. Bioeng., 7: 292–295
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Anton, F., Tappe, A., Kasper, C., Loa, A., Wilhelm, BU., Scheper, T. (2007). The Production of Human Growth Hormone. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_64
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5476-1_64
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)