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Electron Transfer From Ferredoxin and Flavodoxin to Ferredoxin:NADP+ Reductase

  • Chapter
Photosystem I

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 24))

Abstract

An overview is presented of structure/function relationships in the interactions between the small electron transfer proteins ferredoxin (Fd) and flavodoxin (Fld) and the flavoprotein enzyme ferredoxin:NADP+ reductase (FNR), primarily emphasizing the proteins from the cyanobacterium, Anabaena, and the higher plant, spinach. Results are summarized from experiments utilizing rapid-reaction kinetic methods (stopped-flow spectrophotometry and laser flash photolysis) involving wild-type and site-specific mutants of these proteins, redox potential determinations, and X-ray crystallography, including the crystal structure of a Fd/FNR complex. These have provided detailed insights into the protein–protein recognition and electron transfer mechanisms utilized by these systems. Fd and Fld bind to FNR within a concave region of the FNR surface that contains the exposed dimethylbenzene ring of the FAD cofactor. In the Fd case, electron transfer between the iron–sulfur and flavin centers proceeds with a maximum rate constant of 5,500 sec−1 via a direct outer-sphere mechanism. Both electrostatic and hydrophobic interactions occur between the proteins, resulting in a precise surface complementarity.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ, 85721, USA

    John K. Hurley & Gordon Tollin

  2. Departmento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Zaragoza, 50009, Spain

    Milagros Medina & Carlos Gómez-Moreno

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  1. John K. Hurley
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  2. Gordon Tollin
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  3. Milagros Medina
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  4. Carlos Gómez-Moreno
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  1. The Pennsylvania State University, Pennsylvania, USA

    John H. Golbeck

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Hurley, J.K., Tollin, G., Medina, M., Gómez-Moreno, C. (2006). Electron Transfer From Ferredoxin and Flavodoxin to Ferredoxin:NADP+ Reductase. In: Golbeck, J.H. (eds) Photosystem I. Advances in Photosynthesis and Respiration, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4256-0_27

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