Abstract
Nontargeted single-cell proteomics analysis by mass spectrometry with sample multiplexing utilizing isobaric labeling is often performed using a carrier proteome. The presented protocol describes a targeted approach that replaces the carrier proteome with a set of synthetic peptides from selected proteins, which improves the identification and quantification of these proteins in single human cells.
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Acknowledgments
This work was supported by the Swedish Research Council (grant VR 2018-06156).
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Naval, P., Pañeda, L.P., Athanasopoulou, M., Teppo, J.S., Zubarev, R.A., Végvári, Á. (2024). EquiCP: Targeted Single-Cell Proteomics by Mass Spectrometry with Isobaric Labeled Multiplexing. In: Vegvari, A., Teppo, J., Zubarev, R.A. (eds) Mass Spectrometry Based Single Cell Proteomics. Methods in Molecular Biology, vol 2817. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3934-4_11
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DOI: https://doi.org/10.1007/978-1-0716-3934-4_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3933-7
Online ISBN: 978-1-0716-3934-4
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