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Quantitative Analysis of Epigenetic Modifications in Fagopyrum Nuclei with Confocal Microscope, ImageJ, and R Studio

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Buckwheat

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2791))

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Abstract

Epigenetic programming plays a vital role in regulating pluripotency genes, which become activated or inactivated during the processes of dedifferentiation and differentiation during an organism’s development. The analysis of epigenetic modifications has become possible through the technique of immunostaining, where specific antibodies allow the identification of a single target protein. This chapter describes a detailed protocol for the analysis of the epigenetic modifications with the use of confocal microscopy, subsequent image, and statistical analysis on the example of Fagopyrum calli with the use of nine antibodies raised against histone H3 and H4 methylation and acetylation on several lysines as well as DNA methylation.

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Acknowledgments

This work was supported by the National Science Centre Poland, project OPUS19 grant number 2020/37/B/NZ9/01499.

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Authors and Affiliations

  1. Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland

    Alicja Tomasiak, Katarzyna Sala & Agnieszka Braszewska

  2. Institute of Plant Sciences, University of Bern, Bern, Switzerland

    Lea Sophie Berg

Authors
  1. Alicja Tomasiak
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  2. Lea Sophie Berg
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  3. Katarzyna Sala
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  4. Agnieszka Braszewska
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Corresponding author

Correspondence to Agnieszka Braszewska .

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Editors and Affiliations

  1. Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland

    Alexander Betekhtin

  2. Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland

    Artur Pinski

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Tomasiak, A., Berg, L.S., Sala, K., Braszewska, A. (2024). Quantitative Analysis of Epigenetic Modifications in Fagopyrum Nuclei with Confocal Microscope, ImageJ, and R Studio. In: Betekhtin, A., Pinski, A. (eds) Buckwheat. Methods in Molecular Biology, vol 2791. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3794-4_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3794-4_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3793-7

  • Online ISBN: 978-1-0716-3794-4

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