Abstract
Autoimmune responses are characterized by the presence of antibodies and lymphocytes specific to self or so-called autoantigens. Among such autoantigens is DNA; therefore, screening for antibodies recognizing single- and/or double-stranded DNA is commonly used to detect and classify autoimmune diseases. While autoimmunity affects both sexes, females are generally more affected than males, which is recapitulated in some animal models. A variety of factors, including genetic predisposition and the environment, contribute to the development of autoimmune disorders. Since certain drug products may also contribute to the development of autoimmunity, understanding a drug’s potential to trigger an autoimmune response is of interest to immunotoxicology. However, models to study autoimmunity are limited, and it is generally agreed that no model can accurately predict autoimmunity in humans. Herein, we present an in vivo protocol utilizing the SJL/J mouse model to study nanoparticles’ effects on the development of autoimmune responses. The protocol is adapted from the literature describing the use of this model to study chemically induced lupus.
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Acknowledgments
This project has been funded in whole or in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract 75N91019D00024. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government. All animal procedures have been approved by the NCI-Frederick ACUC. NCI-Frederick is accredited by Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International and follows the Public Health Service Policy for the Care and Use of Laboratory Animals. Animal care was provided in accordance with the procedures outlined in the “Guide for Care and Use of Laboratory Animals” (National Research Council; 1996; National Academy Press; Washington, DC).
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Neun, B.W., Potter, T.M., Robinson, C., Difilippantonio, S., Edmondson, E., Dobrovolskaia, M.A. (2024). Analysis of Nanoparticles’ Potential to Induce Autoimmunity. In: Clogston, J.D., Crist, R.M., Dobrovolskaia, M.A., Stern, S.T. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 2789. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3786-9_12
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DOI: https://doi.org/10.1007/978-1-0716-3786-9_12
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3785-2
Online ISBN: 978-1-0716-3786-9
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