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Root Hair Imaging Using Confocal Microscopy

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Plant Functional Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2787))

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Abstract

Plant genetics plays a key role in determining root hair initiation and development. A complex network of genetic interactions therefore closely monitors and influences root hair phenotype and morphology. The significance of these genes can be studied by employing, for instance, loss-of-function mutants, overexpression plant lines, and fluorescently labeled constructs. Confocal laser scanning microscopy is a great tool to visually observe and document these morphological features. This chapter elaborates the techniques involved in handling of microscopic setup to acquire images displaying root hair distribution along the fully elongated zone of Arabidopsis thaliana roots. Additionally, we illustrate an approach to visualize early fate determination of epidermal cells in the root apical meristem, by describing a method for imaging YFP tagged transgenic plant lines.

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Acknowledgments

This work was supported by grants from the Austrian Science Fund (FWF, V658 and P35036).

Author information

Authors and Affiliations

  1. Institute of Molecular Plant Biology, Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria

    Krishna Vasant Mutanwad & Doris Lucyshyn

  2. Core Facility Multiscale Imaging, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria

    Monika Debreczeny

Authors
  1. Krishna Vasant Mutanwad
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  2. Monika Debreczeny
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  3. Doris Lucyshyn
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Corresponding author

Correspondence to Doris Lucyshyn .

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Editors and Affiliations

  1. Plant Functional Genomics Lab, Institute of Molecular Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria

    Fatemeh Maghuly

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mutanwad, K.V., Debreczeny, M., Lucyshyn, D. (2024). Root Hair Imaging Using Confocal Microscopy. In: Maghuly, F. (eds) Plant Functional Genomics. Methods in Molecular Biology, vol 2787. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3778-4_5

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  • DOI: https://doi.org/10.1007/978-1-0716-3778-4_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3777-7

  • Online ISBN: 978-1-0716-3778-4

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