Skip to main content
SpringerLink
Log in

Adipose-Derived Stem Cells

Methods and Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Jeffrey Gimble

    1. Obatala Sciences Inc, New Orleans, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Bruce Bunnell

    1. Department of Microbiology, Immunology and Genetics, University of North Texas Health Science, Fort Worth, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Trivia Frazier

    1. Obatala Sciences Inc, New Orleans, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  4. Cecilia Sanchez

    1. Obatala Sciences Inc, New Orleans, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2783)

  • 4166 Accesses

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Methods to Isolate, Characterize, and Differentiate Human ASC

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Isolation of Human Adipose-Derived Stem Cells

      • Elizabeth Montano, Sara Al-Ghadban, Bruce A. Bunnell
      Pages 3-11

    3. Isolation of Human Adipose-Derived Stromal/Stem Cells from Lipoaspirates

      • Yuejia Li, Ziyi Mei, Jie Li, Jeffrey M. Gimble
      Pages 13-24

    4. Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry

      • Neelima Thottappillil, Mario A. Gomez-Salazar, Mary Archer, Bruno Péault, Aaron W. James
      Pages 25-33

    5. Soft Tissue Reconstruction

      • Mario Alessandri-Bonetti, Francesco M. Egro, Kacey G. Marra
      Pages 35-52

    6. Adult Stem Cells Freezing Processes and Cryopreservation Protocols

      • Mohan Kumar Dey, Ram V. Devireddy
      Pages 53-89

  3. Animal ASC Models

    1. Front Matter

      Pages 91-91
      Download chapter PDF

    2. Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic and Osteogenic Differentiation or Flow Cytometry-Based Analysis

      • Matthew C. Scott, Chul-Hong Park, Marilyn Dietrich, Xiying Wu, Jeffrey M. Gimble, Carrie M. Elks et al.
      Pages 93-107

    3. Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method

      • Ziyi Mei, Yuejia Li, Jeffrey M. Gimble, Jie Li
      Pages 109-114

    4. Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation, Characterization, and Differentiation

      • Takashi Taguchi, Mandi J. Lopez
      Pages 115-136

    5. Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation

      • Catherine Takawira, Wei Duan, Takashi Taguchi, Mandi J. Lopez
      Pages 137-156

  4. Hydrogels, Scaffolds, and 3 Dimensional Microphysiological Systems

    1. Front Matter

      Pages 157-157
      Download chapter PDF

    2. Manufacturing of a Human Adipose-Derived Hydrogel

      • Jordan Robinson, Haley Lassiter, Katie Hamel, Xiying Wu, Jeffrey M. Gimble, Trivia Frazier et al.
      Pages 159-165

    3. Quality Control Assessment of Human Adipose-Derived Hydrogels

      • Haley Lassiter, Jordan Robinson, Katie Hamel, Jeffrey M. Gimble, Trivia Frazier, Cecilia Sanchez
      Pages 167-176

    4. Analysis of Biomechanical Properties of Adipose-Derived Hydrogels for Adipose-Derived Stromal/Stem Cell-Based Hydrogel Culture

      • Jorge A. Belgodere
      Pages 177-193

    5. Combination of Adipose-Derived Stromal/Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications

      • Kainat Ahmed, Haadia Tauseef, Omair A. Mohiuddin
      Pages 195-207

    6. Preparation of Decellularized Amniotic Membrane and Adipose-Derived Stromal/Stem Cell Seeding

      • Haadia Tauseef, Kainat Ahmed, Asmat Salim, Omair A. Mohiuddin
      Pages 209-220

    7. Robust Generation of ASC Spheroids for Use as 3D Cultures and in Bioprinted Tissue Models

      • Martin Watzling, Hannes Horder, Petra Bauer-Kreisel, Torsten Blunk
      Pages 221-233

    8. Generating and Characterizing Adipose Spheroids from Adipose-Derived Stromal/Stem Cells

      • Charles Amurgis, W. Vincent Nerone, Lauren Kokai
      Pages 235-262
Back to top

Keywords

About this book

Since the publication of the previous editions, there has been increased focus on the use of adipose-derived stromal/stem cells (ASC) and stromal vascular fraction (SVF) cells in three-dimensional hydrogel-based scaffolds for the development of microphysiological systems (MPS) serving as in vitro humanized assays and alternatives to in vivo pre-clinical animal models. This third edition volume discusses of the latest technology and advancements in the field of human-derived ASC and SVF. The chapters in this book are organized into four parts. Part One focuses on human ASC’s isolation, characterization, and differentiation. Part Two describes the isolation and characterization of ASC and SVF from canine, feline, and murine tissues. Part Three looks at hydrogels, scaffolds, and microphysiological systems, and Part Four talks about the new assays and applications using ASC. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Adipose-Derived Stem Cells: Methods and Protocols, Third Edition is a valuable resource for both novice and experts researchers interested in learning more about this important and developing field.  


Editors and Affiliations

  • Obatala Sciences Inc, New Orleans, USA

    Jeffrey Gimble, Trivia Frazier, Cecilia Sanchez

  • Department of Microbiology, Immunology and Genetics, University of North Texas Health Science, Fort Worth, USA

    Bruce Bunnell

Bibliographic Information

  • Book Title: Adipose-Derived Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Jeffrey Gimble, Bruce Bunnell, Trivia Frazier, Cecilia Sanchez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3762-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3761-6Published: 14 March 2024

  • Softcover ISBN: 978-1-0716-3764-7Due: 14 April 2024

  • eBook ISBN: 978-1-0716-3762-3Published: 13 March 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XV, 393

  • Number of Illustrations: 22 b/w illustrations, 69 illustrations in colour

  • Topics: Stem Cells, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation