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Culture of Human Fetal Membranes in a Two Independent Compartment Model: An Ex Vivo Approach

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Maternal Placental Interface

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2781))

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Abstract

During pregnancy, the fetal membranes composed of the amnion and chorodecidua constitute a selective barrier separating two distinct environments, maternal and fetal. These tissues have the function of delimiting the amniotic cavity. Their histological complexity gives them physical, mechanical, and immunological properties to protect the fetus. Although the study of the amnion, chorion, and decidua separately provides knowledge about the functions of the fetal membranes, the protocol we describe in this chapter has the advantage of maintaining the biological and functional complexity of these tissues. In addition, this experimental model allows the researcher to recreate various pathological scenarios because this model allows for differential stimulation of the amnion or choriodecidua.

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Acknowledgments

We thank the microbiology and pathology department of the Instituto Nacional de Perinatología for their support in obtaining and isolating the bacterial strains, as well as Dr. Pilar Velazquez of Hospital Ángeles México for her support in getting biological samples for the standardization of the protocol.

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Authors and Affiliations

  1. Department of Immunobiochemistry, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico

    Pilar Flores-Espinosa, Andrea Olmos-Ortiz & Verónica Zaga-Clavellina

  2. Department of Infectology and Immunology, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico

    Ismael Mancilla-Herrera

  3. Departamento de Biología de la Reproducción Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico

    Lorenza Díaz

Authors
  1. Pilar Flores-Espinosa
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  2. Ismael Mancilla-Herrera
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  3. Andrea Olmos-Ortiz
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  4. Lorenza Díaz
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  5. Verónica Zaga-Clavellina
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Corresponding author

Correspondence to Verónica Zaga-Clavellina .

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Editors and Affiliations

  1. Department of Immunobiochemistry, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico

    Verónica Zaga-Clavellina

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Flores-Espinosa, P., Mancilla-Herrera, I., Olmos-Ortiz, A., Díaz, L., Zaga-Clavellina, V. (2024). Culture of Human Fetal Membranes in a Two Independent Compartment Model: An Ex Vivo Approach. In: Zaga-Clavellina, V. (eds) Maternal Placental Interface. Methods in Molecular Biology, vol 2781. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3746-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3746-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3745-6

  • Online ISBN: 978-1-0716-3746-3

  • eBook Packages: Springer Protocols

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