Abstract
The immunofluorescence technique has been used to identify pluripotent markers in the human amniotic epithelial cells (hAEC). hAEC belonging to human fetal membranes, specificamently to amnion layer, and are arising by epiblast, this sugest that the hAEC have characteristics of epiblast cells, in other words, characteristcs of pluripotent stem cells. Here we describe obtaining human amnion tissue and identifying pluripotent markers by immunofluorescence.
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Funding and Acknowledgments
We thank Gynecology and Obstetrics resident Mendoza Ríos-Edgar Iván, M.D., (Secretaría de Salud) for his great help in samples collection. We thank the students Escudero Medina-Elías Adrián (Universidad Juárez Autónoma de Tabasco) and Salazar Alonzo-Jonathan (Universidad Autónoma Metropolitana Unidad Cuajimalpa) for their excellent assistance in the Laboratory of Physiology and Cell Development at INPer.
Our research was supported by grants from Instituto Nacional de Perinatología de México (2018–1-150 and 2021-01-24).
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Martínez-Herrera, S.C. et al. (2024). Obtaining Tissues of Human Amniotic Membrane and Identification of Pluripotent Markers. In: Zaga-Clavellina, V. (eds) Maternal Placental Interface. Methods in Molecular Biology, vol 2781. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3746-3_15
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DOI: https://doi.org/10.1007/978-1-0716-3746-3_15
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