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Transmembrane β-Barrel Proteins

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Raffaele Ieva

    1. Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, CNRS, Université de Toulouse, Toulouse, France

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2778)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Name Is Barrel, β-Barrel

    • Scout Hayashi, Susan K. Buchanan, Istvan Botos
    Pages 1-30

  3. Recombinant Expression and Overproduction of Transmembrane β-Barrel Proteins

    • Ina Meuskens, Jack C. Leo, Dirk Linke
    Pages 31-41

  4. Bacterial Envelope Fractionation

    • Athanasios Saragliadis, Dirk Linke
    Pages 43-52

  5. Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System

    • Rachael Duodu, Dirk Linke, Jack C. Leo
    Pages 53-63

  6. In Vitro Reconstruction of Bacterial β-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane

    • Eriko Aoki, Edward Germany, Takuya Shiota
    Pages 65-81

  7. Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes

    • Minh Sang Huynh, Jiaming Caitlyn Xu, Trevor F. Moraes
    Pages 83-99

  8. In Vivo Disulfide-Bond Crosslinking to Study β-Barrel Membrane Protein Interactions, Dynamicity, and Folding Intermediates

    • Matthew Thomas Doyle
    Pages 101-115

  9. Site-Specific Photocrosslinking to Investigate Toxin Delivery Mediated by the Bacterial β-Barrel Assembly Machine

    • Emily M. Bouzan, Christine L. Hagan
    Pages 117-131

  10. Analysis of Transmembrane β-Barrel Proteins by Native and Semi-native Polyacrylamide Gel Electrophoresis

    • Violette Morales, Luis Orenday-Tapia, Raffaele Ieva
    Pages 133-145

  11. Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs

    • Zhiyu Zhao, John William Young, Franck Duong van hoa
    Pages 147-158

  12. Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery

    • Federico Corona, Waldemar Vollmer
    Pages 159-183

  13. MitoLuc: A Luminescence-Based Assay to Study Real-Time Protein Import into Mitochondria

    • Holly C. Ford, Ian Collinson
    Pages 185-200

  14. Modular Assembly of Mitochondrial β-Barrel Proteins

    • Rituparna Bhowmik, Fabian den Brave, Thomas Becker
    Pages 201-220

  15. Tracking the Activity and Position of Mitochondrial β-Barrel Proteins

    • Shuo Wang, Stephan Nussberger
    Pages 221-236

  16. Conformational Heterogeneity of β-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy

    • Sophie Ketter, Aathira Gopinath, Benesh Joseph
    Pages 237-257

  17. Characterization of β-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry

    • Antonio N. Calabrese
    Pages 259-272

  18. Dissecting the Organization of a β-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry

    • Xiangfeng Lai, Hsin-Hui Shen
    Pages 273-290

  19. Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel

    • Rebecca Conners, Mathew McLaren, Marjorie Russel, Vicki A. M. Gold
    Pages 291-310

  20. Recent Advances in Modeling Membrane β-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies

    • Anna L. Duncan, Ya Gao, Evert Haanappel, Wonpil Im, Matthieu Chavent
    Pages 311-330
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Keywords

About this book

This detailed volume explores experimental strategies and protocols for the expression, assembly, characterization, and exploitation of transmembrane β-barrel proteins. Beginning with methodologies to study their assembly, the book continues with protocols for characterizing the landscape of transmembrane β-barrel protein interactions with other cellular factors, dissecting processes of protein transport in bacteria and mitochondria, examining structural characterization, determination, and prediction, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Transmembrane β-Barrel Proteins: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of these vital membrane-spanning proteins.

Editors and Affiliations

  • Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, CNRS, Université de Toulouse, Toulouse, France

    Raffaele Ieva

Bibliographic Information

  • Book Title: Transmembrane β-Barrel Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Raffaele Ieva

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3734-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3733-3Published: 14 March 2024

  • Softcover ISBN: 978-1-0716-3736-4Due: 14 April 2024

  • eBook ISBN: 978-1-0716-3734-0Published: 13 March 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 386

  • Number of Illustrations: 21 b/w illustrations, 54 illustrations in colour

  • Topics: Membrane Biology, Chemistry/Food Science, general

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