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Mimicking the Tumor Niche: Methods for Isolation, Culture, and Characterization of Cancer Stem Cells and Multicellular Spheroids

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Cancer Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2777))

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Abstract

Cancer stem cells (CSCs) play a significant role in driving several tumor hallmarks. Their behavior and tumor progression are strictly related to the tumor microenvironment (TME). The dynamic interplay between CSCs and TME drives metastasis, chemoresistance, and disease relapse. In this chapter, we describe different techniques and protocols for isolating, culturing, and characterizing CSCs and we explain the methodology for the culture of multicellular spheroids comprising CSCs.

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Author information

Authors and Affiliations

  1. Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain

    Laura De Lara-Peña, Julia López de Andrés, Carmen Griñán-Lisón & Juan Antonio Marchal

  2. Instituto de Investigación Biosanitaria ibs.GRANADA, University Hospitals of Granada-University of Granada, Granada, Spain

    Laura De Lara-Peña, Julia López de Andrés, Carmen Griñán-Lisón & Juan Antonio Marchal

  3. Excellence Research Unit ‘Modeling Nature’ (MNat), University of Granada, Granada, Spain

    Laura De Lara-Peña, Julia López de Andrés, Carmen Griñán-Lisón & Juan Antonio Marchal

  4. Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, Granada, Spain

    Laura De Lara-Peña, Julia López de Andrés & Juan Antonio Marchal

  5. BioFab i3D Lab- Biofabrication and 3D (bio)printing Singular Laboratory, University of Granada, Granada, Spain

    Laura De Lara-Peña, Julia López de Andrés, Carmen Griñán-Lisón & Juan Antonio Marchal

  6. Department of Biomedical Sciences-Histology, University of Sassari, Sassari, Italy

    Cristiano Farace, Andrea Pisano, Grazia Fenu, Federica Etzi & Roberto Madeddu

  7. I.N.B.B. National Institute of Biostructure and Biosystem, Rome, Italy

    Cristiano Farace, Federica Etzi & Roberto Madeddu

  8. Department of Biochemistry and Molecular Biology 2, School of Pharmacy, University of Granada, Granada, Spain

    Carmen Griñán-Lisón

  9. GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, Granada, Spain

    Carmen Griñán-Lisón

Authors
  1. Laura De Lara-Peña
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  2. Cristiano Farace
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  3. Andrea Pisano
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  4. Julia López de Andrés
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  5. Grazia Fenu
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  6. Federica Etzi
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  7. Carmen Griñán-Lisón
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  8. Juan Antonio Marchal
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  9. Roberto Madeddu
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Corresponding authors

Correspondence to Juan Antonio Marchal or Roberto Madeddu .

Editor information

Editors and Affiliations

  1. Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, Baronissi, Italy

    Federica Papaccio

  2. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianpaolo Papaccio

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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De Lara-Peña, L. et al. (2024). Mimicking the Tumor Niche: Methods for Isolation, Culture, and Characterization of Cancer Stem Cells and Multicellular Spheroids. In: Papaccio, F., Papaccio, G. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 2777. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3730-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3730-2_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3729-6

  • Online ISBN: 978-1-0716-3730-2

  • eBook Packages: Springer Protocols

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