Abstract
Radiotherapy is a widely used approach for cancer treatment. However, delivering a single high dose of radiation to bulky tumors can be challenging due to the toxicities induced in the surrounding healthy tissue. To overcome this issue, a nonuniform high dose can be delivered using partial-volume tumor irradiation or spatially fractionated radiotherapy (SFRT). Moreover, SFRT has the potential to induce a stronger antitumor immune response compared to traditional radiotherapy due to the preservation of immune cells in the unirradiated tumor regions. There are several SFRT approaches, including GRID therapy, three-dimensional GRID therapy (LATTICE), microbeam radiation therapy (MRT), and Stereotactic Body Radiation Therapy for PArtial Tumor irradiation targeting exclusively the HYpoxic segment (SBRT-PATHY). The following protocol describes partial-volume tumor irradiation, a technique that enables dose delivery to only a part of the tumor in mice using an X-ray generator and collimators of different dimensions that limit the size of the irradiation field.
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The authors acknowledge financial support from the Slovenian Research Agency under grant number P3-0003 and postgraduate research funding.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Modic, Z., Markelc, B., Jesenko, T. (2024). Partial-Volume Irradiation of Murine Tumors. In: Čemažar, M., Jesenko, T., Lampreht Tratar, U. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 2773. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3714-2_10
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DOI: https://doi.org/10.1007/978-1-0716-3714-2_10
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