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Preparation of ER Microsomes from Arabidopsis thaliana

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The Plant Endoplasmic Reticulum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2772))

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Abstract

Microsomes are vesicles derived from the endoplasmic reticulum (ER) when cells are broken down in the lab. These microsomes are a valuable tool to study a variety of ER functions such as protein and lipid synthesis in vitro.

Here we describe a protocol to isolate ER-derived microsomes Arabidopsis thaliana seedlings and exemplify the use of these purified microsomes in enzyme assays with the auxin precursors tryptophan (Trp) or indole-3-pyruvic acid (IPyA) to quantify auxin synthetic capacity in microsomal and cytosolic fractions.

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Acknowledgments

This work was supported by a research scholarship from the Korean Federation of Science and Technology Societies (KOFST) awarded to Dr. Verena Kriechbaumer and the British Biotechnology and Biological Sciences Research Council (grant no. BB/J004987/1 research grant) awarded to CH.

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Authors and Affiliations

  1. Endomembrane Structure and Function Research Group, Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK

    Verena Kriechbaumer

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  1. Verena Kriechbaumer
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Correspondence to Verena Kriechbaumer .

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Editors and Affiliations

  1. Biological and Medical Sciences, Oxford Brookes University, Oxford, UK

    Verena Kriechbaumer

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kriechbaumer, V. (2024). Preparation of ER Microsomes from Arabidopsis thaliana. In: Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum. Methods in Molecular Biology, vol 2772. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3710-4_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3710-4_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3709-8

  • Online ISBN: 978-1-0716-3710-4

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