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3D Cell Culture

Methods and Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Zuzana Sumbalova Koledova

    1. Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2764)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. 3D Cell Culture: Techniques For and Beyond Organoid Applications

    • Zuzana Sumbalova Koledova
    Pages 1-12

  3. Hydrogels and Scaffolds for 3D Cell Culture

    1. Front Matter

      Pages 13-13
      Download chapter PDF

    2. Preparation of Corneal Tissue Matrix Bioink for 3D Bioprinting

      • Shibu Chameettachal, Falguni Pati
      Pages 15-20

    3. Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering

      • Katarína Čimborová, Hana Kotasová, Vendula Pelková, Veronika Sedláková, Aleš Hampl
      Pages 21-33

    4. Three-Dimensional Hanging Drop Spheroid Plates for Easy Chimeric Antigen Receptor (CAR) T Cytotoxicity Assay

      • Zhenzhong Chen, Sungsu Park
      Pages 35-42

    5. Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds

      • Carolina Oliver-Urrutia, Anna Diez-Escudero, Zuzana Sumbalova Koledova, Ladislav Čelko, Nils P. Hailer, Edgar B. Montufar
      Pages 43-60

    6. Modeling Acute Myeloid Leukemia Using StarPEG-Heparin Hydrogels

      • P. Lewen Holloway, Akhilandeshwari Ravichandran, Julien Clegg, Claudia Bruedigam, Laura J. Bray
      Pages 61-73

  4. 3D Organoid and Organotypic Cultures

    1. Front Matter

      Pages 75-75
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    2. A Decision Tree to Guide Human and Mouse Mammary Organoid Model Selection

      • Marika Caruso, Kamyab Saberiseyedabad, Larissa Mourao, Colinda L. G. J. Scheele
      Pages 77-105

    3. Fibroblast-Epithelium Co-culture Methods Using Epithelial Organoids and Cell Line–Derived Spheroids

      • Jakub Sumbal, Zuzana Sumbalova Koledova
      Pages 107-129

    4. Differentiation of Fibroblasts to Adipocytes in 3D for a Co-culture with Mammary Organoids and Immunohistological Analysis

      • Matea Brezak, Lukas Kubec, Zuzana Sumbalova Koledova
      Pages 131-144

    5. Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland

      • Alison Kuony, Matea Brezak, René-Marc Mège, Zuzana Sumbalova Koledova
      Pages 145-156

    6. Renal Organoids from Whole Kidney Cells

      • Liang Chen
      Pages 157-164

    7. Organotypic 3D Ex Vivo Co-culture Model of the Macro-metastasis/Organ Parenchyma Interface

      • Raquel Blazquez, Daniela Sparrer, Jessica Sonbol, Jürgen Philipp, Florian Schmieder, Tobias Pukrop
      Pages 165-176

  5. Functional Experiments in 3D Cell Cultures

    1. Front Matter

      Pages 177-177
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    2. Addressing the Mechanical Interaction Between Cancer-Associated Fibroblasts and Cancer Cells by Laser Ablation

      • Carlos Perez-Gonzalez, Jorge Barbazán
      Pages 179-203

    3. Double-Barrel Perfusion System for Modification of Luminal Contents of Intestinal Organoids

      • Nicholas J. Ginga, Raleigh Slyman
      Pages 205-224

    4. Mechanical Actuation of Organoids in Synthetic Microenvironments

      • Francesca Sgualdino, Lorenzo Mattolini, Brian Daza Jimenez, Kieran Patrick, Abdel Rahman Abdel Fattah, Adrian Ranga
      Pages 225-245

  6. Microfluidic and Bioprinting Approaches for 3D Cell Culture

    1. Front Matter

      Pages 247-247
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Keywords

About this book

Since the publication of the previous edition, the use of 3D cell and organoid cultures has become more widespread across laboratories. This second edition volume expands on the previous edition with discussions about the latest organoid models developed for many more organs; new hydrogels and devices for 3D culture; and the organoid systems that have been improved by incorporating more components of tissue microenvironments in the in vitro culture. The chapters in this book are organized into five parts and cover topics such as biofabrication, organoids, microfluidic systems, bioprinting, and image analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Thorough and cutting-edge, 3D Cell Culture: Methods and Protocols, Second Edition is a valuable resource that will stimulate new ideas, foster interdisciplinary collaborations, and contribute to the improvement of human health and well-being.


Editors and Affiliations

  • Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic

    Zuzana Sumbalova Koledova

Bibliographic Information

  • Book Title: 3D Cell Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Zuzana Sumbalova Koledova

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3674-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3673-2Published: 24 February 2024

  • Softcover ISBN: 978-1-0716-3676-3Due: 26 March 2024

  • eBook ISBN: 978-1-0716-3674-9Published: 23 February 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 336

  • Number of Illustrations: 5 b/w illustrations, 77 illustrations in colour

  • Topics: Biological Techniques, Cell Biology

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