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High-Resolution Respirometry for Mitochondrial Function in Rodent Brain

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Neuroprotection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2761))

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Abstract

High-resolution mitochondrial respirometry is a modern technique that enables to measure mitochondrial respiration in various cell types. It contains chambers with oxygen sensors that measure oxygen concentration via polarography and calculate its consumption. The chamber contains plastic stoppers with injection ports that allow the injection of samples and different substrates, inhibitors, and uncoupler substances to measure mitochondrial respiration with high efficiency. These substances act on the mitochondrial electron transport chain (ETC) and help to assess the mitochondrial ATP production capacity and oxidative phosphorylation. The respirograph obtained with the help of software represents the oxygen consumption in each stage after adding different reagents.

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Acknowledgments

Authors acknowledge the Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, Government of India; National Institute of Pharmaceutical Education and Research (NIPER)−Ahmedabad; Indian Council of Medical Research (ICMR), New Delhi, for the senior research fellowship grant to Ms. Aishika Datta (45/13/2020-PHA/BMS); as well as the NanoBio project grant to Dr. Pallab Bhattacharya (34/5/2019-TF/Nano/BMS). We also acknowledge Oroboros Instruments (Oxygraph-2k) and the experimental protocols provided by them which have been followed with certain modifications at our lab.

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Ahmedabad, Gandhinagar, Gujarat, India

    Aishika Datta, Deepaneeta Sarmah, Bijoyani Ghosh, Nikita Rana & Pallab Bhattacharya

  2. Cellular and Molecular Neurobiology Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India

    Anupom Borah

Authors
  1. Aishika Datta
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  2. Deepaneeta Sarmah
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  3. Bijoyani Ghosh
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  4. Nikita Rana
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  5. Anupom Borah
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  6. Pallab Bhattacharya
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Corresponding author

Correspondence to Pallab Bhattacharya .

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Editors and Affiliations

  1. Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA

    Swapan K. Ray

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Datta, A., Sarmah, D., Ghosh, B., Rana, N., Borah, A., Bhattacharya, P. (2024). High-Resolution Respirometry for Mitochondrial Function in Rodent Brain. In: Ray, S.K. (eds) Neuroprotection. Methods in Molecular Biology, vol 2761. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3662-6_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3662-6_4

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3661-9

  • Online ISBN: 978-1-0716-3662-6

  • eBook Packages: Springer Protocols

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