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Borrelia burgdorferi

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Leona Gilbert

    1. Tezted Ltd, Jyväskylä, Finland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2742)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
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  2. Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-borne Pathogens in 48 or 96 Samples

    • Sara Moutailler, Clemence Galon
    Pages 1-17

  3. Direct Detection of Borrelia Species in Tissues

    • Shiva Kumar Goud Gadila, Monica E. Embers
    Pages 19-35

  4. Purification of Borrelia burgdorferi Outer Membrane Vesicles

    • Hanna Tammisto, Kati Karvonen
    Pages 37-45

  5. Constructing an ELISA for Detection of Anti-Borrelia in Wildlife and Agricultural Animals

    • Julia Bland, Caitlin McGowan, Emma Bush, Vett Lloyd
    Pages 47-67

  6. A Step-by-Step Guide to Selecting an Optimal Cut-Off Value Based on the Receiver Operating Characteristic and Youden Index in Methods Designed to Diagnose Lyme Disease

    • Kunal Garg, Sara Campolonghi
    Pages 69-76

  7. Adaptive Immune Response Investigation in Lyme Borreliosis

    • Mihail Pruteanu, Armin Schwarzbach, Markus Berger
    Pages 77-90

  8. CYTOSPOT: Intracellular Staining and Detection of Cytokines by Flow Cytometer, an Alternative to LYMESPOT

    • Luis Calderilla-Barbosa, Jose Luis Flores-Sevilla
    Pages 91-98

  9. Use of Specific Borrelia Phages as a New Strategy for Improved Diagnostic Tests

    • Jinyu Shan, Ying Jia, Tatjana Mijatovic
    Pages 99-104

  10. Identification of Unambiguous Borrelia Peptides in Human Urine Using Affinity Capture and Mass Spectrometry

    • Rocio Cornero, Sumaiya Safia Irfan, Silvia Cachaco, Weidong Zhou, Ahana Byne, Marissa Howard et al.
    Pages 105-122

  11. Preparation of Borrelia-Infected Mammalian Cells for Helium Ion Microscopy

    • Kati Karvonen
    Pages 123-129

  12. Establishing a Zebrafish Model for Borrelia burgdorferi Infection Using Immersion and Microinjection Methods

    • Erica Misner, Min Zhang, Eva Sapi
    Pages 131-149

  13. Development of Organoids to Study Infectious Host Interactions

    • Aparajita Lahree, Leona Gilbert
    Pages 151-164

  14. Content Analysis: An Approach to Exploring the Depiction of Tick-Borne Diseases

    • Stephen M. Croucher
    Pages 165-172

  15. Applying BERT and ChatGPT for Sentiment Analysis of Lyme Disease in Scientific Literature

    • Teo Susnjak
    Pages 173-183

  16. Building a Binary Classification Machine-Learning Model: A Guide to Predicting Participation in a Lyme Disease Program at a Medical Institute

    • Kunal Garg, Liria Mitzuko Fajardo-Yamamoto, Flor Cecilia Rojas-Castro, Teo Susnjak, Leona Gilbert
    Pages 185-237

  17. Clinical Algorithm and Diagnostic Tools for Lyme Disease

    • Gisell García-Bretón, Flor C. Rojas-Castro, Astrid N. Espino-Vázquez, Liria M. Fajardo-Yamamoto
    Pages 239-243

  18. Establishing a Pregnancy Lyme Disease Biobank

    • Stanley J. Naides
    Pages 245-257

  19. Developing a Prospective Gestational Lyme Disease Study

    • Graham McLennan, Suzanne E. Dale, Laura Gillim, Vivian Weinblatt, Robert Wallerstein, Stanley J. Naides
    Pages 259-278

  20. Back Matter

    Pages 279-280
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Keywords

About this book

This volume covers the latest advancements and techniques used to understand the fastidious bacterium, Borrelia burgdorferi, and its significance in infectious disorders by combining both conventional and cutting-edge approaches. This book covers diverse topics, including direct detection, diagnostic methods, immune response analysis, alternative model systems, advanced proteomics, social media analysis, and clinical research. It also discusses unconventional wet lab research such as content analysis, the use of ChatGPT, clinical algorithms for chronic Lyme, establishment of a pregnancy Lyme disease biobank, and investigates Lyme in pregnant women. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Borrelia burgdorferi: Methods and Protocols encompasses a wide range of techniques and caters to scientists from various disciplines and career stages, such as cell and molecular biologists, statisticians, and clinical researchers. 


Editors and Affiliations

  • Tezted Ltd, Jyväskylä, Finland

    Leona Gilbert

Bibliographic Information

  • Book Title: Borrelia burgdorferi

  • Book Subtitle: Methods and Protocols

  • Editors: Leona Gilbert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3561-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3560-5Published: 03 January 2024

  • Softcover ISBN: 978-1-0716-3563-6Due: 03 February 2024

  • eBook ISBN: 978-1-0716-3561-2Published: 02 January 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 280

  • Number of Illustrations: 10 b/w illustrations, 42 illustrations in colour

  • Topics: Microbiology, Cell Biology

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