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Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus

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Bacteriophages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2738))

Abstract

Cryogenic electron microscopy (cryo-EM) single-particle analysis has revolutionized the structural analysis of icosahedral viruses, including tailed bacteriophages. In recent years, localized (or focused) reconstruction has emerged as a powerful data analysis method to capture symmetry mismatches and resolve asymmetric features in icosahedral viruses. Here, we describe the methods used to reconstruct the 2.65-MDa tail apparatus of the Shigella phage Sf6, a representative member of the Podoviridae superfamily.

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Acknowledgment

This work was supported by the National Institutes of Health grants R01 GM100888, R35 GM140733, and S10 OD030457 to G.C. This research was partly supported by the National Cancer Institute’s National Cryo-EM Facility at the Frederick National Laboratory for Cancer Research under contract HSSN261200800001E.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA

    Chun-Feng David Hou, Fenglin Li, Stephano Iglesias & Gino Cingolani

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  1. Chun-Feng David Hou
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  2. Fenglin Li
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  3. Stephano Iglesias
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  4. Gino Cingolani
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Corresponding author

Correspondence to Gino Cingolani .

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Editors and Affiliations

  1. School of Veterinary Medicine, Texas Tech University, Amarillo, TX, USA

    Ebenezer Tumban

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Hou, CF.D., Li, F., Iglesias, S., Cingolani, G. (2024). Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus. In: Tumban, E. (eds) Bacteriophages. Methods in Molecular Biology, vol 2738. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3549-0_14

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  • DOI: https://doi.org/10.1007/978-1-0716-3549-0_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3548-3

  • Online ISBN: 978-1-0716-3549-0

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