Skip to main content
SpringerLink
Log in

Reverse Genetics of RNA Viruses

Methods and Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Daniel R. Pérez

    1. College of Veterinary Medicine, University of Georgia, Athens, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2733)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

About this book

This second edition volume expands on the previous edition with new and updated chapters that highlight the latest methods and approaches for the manipulation of RNA viruses.  The chapters in this book explore the fundamental role in studying RNA viruses; identifying markers of host range, disease, and transmission; and aid readers with the further development of in silico computational biology tools and artificial intelligence algorithms that can help predict the emergence of certain pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Reverse Genetics of RNA Viruses: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this developing field.


Similar content being viewed by others

Keywords

Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Reverse Genetics Systems for Filoviruses

    • Bianca S. Bodmer, Thomas Hoenen
    Pages 1-14

  3. Reverse Genetics Systems for the De Novo Rescue of Diverse Members of Paramyxoviridae

    • Griffin Haas, Benhur Lee
    Pages 15-35

  4. Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes

    • Arantza Cobela-García, Ignacio Mena, Adolfo García-Sastre
    Pages 37-46

  5. Use of Reverse Genetics for the Generation of Recombinant Influenza Viruses Carrying Nanoluciferase

    • C. Joaquin Caceres, L. Claire Gay, Flavio Cargnin Faccin, Daniel R. Pérez
    Pages 47-74

  6. Reverse Genetics of Bat Influenza A Viruses

    • Susanne Kessler, Adolfo García-Sastre, Martin Schwemmle, Kevin Ciminski
    Pages 75-86

  7. Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA

    • Daniela Toro-Ascuy, Matías Cárdenas, Yesseny Vásquez-Martínez, Marcelo Cortez-San Martín
    Pages 87-99

  8. Reverse Genetics System for Rift Valley Fever Virus

    • Breanna Tercero, Shinji Makino
    Pages 101-113

  9. Plasmid-Based Lassa Virus Reverse Genetics

    • Luis Martínez-Sobrido, Chengjin Ye, Juan Carlos de la Torre
    Pages 115-131

  10. Bacterial Artificial Chromosome Reverse Genetics Approaches for SARS-CoV-2

    • Kevin Chiem, Aitor Nogales, Fernando Almazán, Chengjin Ye, Luis Martínez-Sobrido
    Pages 133-153

  11. Construction of Infectious Clones for Human Enteroviruses

    • Thinesshwary Yogarajah, Justin Jang Hann Chu
    Pages 155-174

  12. Reverse Genetics of Hepatitis C Virus Using an RNA Polymerase I-Mediated Transcription

    • Ryosuke Suzuki, Tetsuro Suzuki
    Pages 175-183

  13. Reverse Genetics of Zika Virus Using a Bacterial Artificial Chromosome

    • Aitor Nogales, Luis Martínez-Sobrido, Fernando Almazán
    Pages 185-206

  14. A Stable Reverse Genetics System of Zika Virus Based on a Self-Splicing Group II Intron

    • Zhong-Yu Liu, Xiao-Feng Li, Cheng-Feng Qin
    Pages 207-229

  15. Reverse Genetics of Dengue Virus

    • José Valter Joaquim Silva Júnior, Andréa Nazaré Monteiro Rangel da Silva, Jefferson José da Silva Santos, Laura Helena Vega Gonzales Gil
    Pages 231-248

  16. Recovery of Recombinant Rotaviruses by Reverse Genetics

    • Chantal A. Agbemabiese, Asha A. Philip, John T. Patton
    Pages 249-263

  17. Back Matter

    Pages 265-272
    Download chapter PDF
Back to top

Editors and Affiliations

  • College of Veterinary Medicine, University of Georgia, Athens, USA

    Daniel R. Pérez

Bibliographic Information

  • Book Title: Reverse Genetics of RNA Viruses

  • Book Subtitle: Methods and Protocols

  • Editors: Daniel R. Pérez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3533-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3532-2Published: 09 December 2023

  • Softcover ISBN: 978-1-0716-3535-3Due: 09 January 2024

  • eBook ISBN: 978-1-0716-3533-9Published: 08 December 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 272

  • Number of Illustrations: 5 b/w illustrations, 40 illustrations in colour

  • Topics: Virology, Genetics and Genomics

Publish with us

Policies and ethics

Back to top

Search

Navigation