Abstract
To improve our understanding of human placental function and placental cell responses to pregnancy stressors, the development of in vitro models that better recapitulate the in vivo placental microenvironment is needed. Here, we describe a three-dimensional (3D) silicone polymer polydimethylsiloxane (PDMS) microfluidic platform for modeling human trophoblast invasion recreating a placental heterocellular microenvironment. This platform allows the formation of a cellular barrier establishing a chemical gradient and real-time evaluation of trophoblast cell invasion and heterocellular cell-to-cell interactions.
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Funding Source
This publication was supported by the National Institute of Environmental Health Sciences of the National Institute of Health (R01ES027863 to A.V-L and P30ES027792).
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Pu, Y., Veiga-Lopez, A. (2024). A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening. In: Raha, S. (eds) Trophoblasts. Methods in Molecular Biology, vol 2728. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3495-0_18
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DOI: https://doi.org/10.1007/978-1-0716-3495-0_18
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3495-0
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