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Experimental Transmission of Plant Viruses by Aphids or Whiteflies

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Plant-Virus Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2724))

Abstract

Numerous species of plant viruses are naturally transmitted by insect vectors, mainly homopterans like aphids and whiteflies. Depending on the vector specificity and the mode of transmission, different durations of the periods for acquisition, retention, and inoculation are required for a successful transmission. Therefore, the experimental setup to perform controlled transmission experiments under laboratory conditions involves handling the vector organisms and managing the times for the different steps of the process to optimize and standardize the results. This chapter describes some basic procedures that can be applied to vector-mediated transmission experiments with selected viruses using aphids or whiteflies and different host plants.

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Acknowledgments

The authors’ work at CRAG and IHSM has received funding from the European Union’s Horizon 2020 Research and Innovation Program under Grant Agreement No. 101000570, Project VIRTIGATION, and from Grants PID2019-105692RB-100 and CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033, and by the CERCA Programme/Generalitat de Catalunya. I.O. was supported by grant BES-2017-080808 funded by MCIN/AEI/ 10.13039/501100011033 and by “ESF Investing in your future.” The authors thank Clara Ontañón for her assistance in capturing the photographs featured in the figures.

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Authors and Affiliations

  1. Centre for Research in Agricultural Genomics (CRAG, CSIC-IRTA-UAB-UB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain

    Irene Ontiveros & Juan José López-Moya

  2. Institute for Mediterranean and Subtropical Horticulture La Mayora (IHSM), CSIC-UMA, Málaga, Spain

    Irene Ontiveros, Juan Antonio Diaz-Pendón & Juan José López-Moya

Authors
  1. Irene Ontiveros
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  2. Juan Antonio Diaz-Pendón
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  3. Juan José López-Moya
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Corresponding author

Correspondence to Juan José López-Moya .

Editor information

Editors and Affiliations

  1. Departamento de Bioquimica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

    Elizabeth P.B. Fontes

  2. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

    Kristiina Mäkinen

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Ontiveros, I., Diaz-Pendón, J.A., López-Moya, J.J. (2024). Experimental Transmission of Plant Viruses by Aphids or Whiteflies. In: Fontes, E.P., Mäkinen, K. (eds) Plant-Virus Interactions. Methods in Molecular Biology, vol 2724. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3485-1_12

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  • DOI: https://doi.org/10.1007/978-1-0716-3485-1_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3484-4

  • Online ISBN: 978-1-0716-3485-1

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