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Therapeutic Proteins

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Millicent O. Sullivan

    1. Department of Chemical & Biomolecular Engineering, University of Delaware, Newark, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Bryce Chackerian

    1. Department of Molecular Genetics and Microbiology School of Medicine, University of New Mexico, Albuquerque, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Wilfred Chen

    1. Department of Chemical & Biomolecular Engineering, University of Delaware, Newark, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2720)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. Discovery, Production, and Conjugation of Therapeutic Proteins

    1. Front Matter

      Pages 1-1
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    2. Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production

      • Tejaswini Ramakrishna Hegde, Ogechi Okocha Rufus, Joongoo Lee, Seok Hoon Hong
      Pages 3-16

    3. Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains

      • Seth D. Ludwig, Angela Zhu, Ankith P. Maremanda, Helen M. Dooley, Jamie B. Spangler
      Pages 17-33

    4. Selective and Site-Specific Incorporation of Nonstandard Amino Acids Within Proteins for Therapeutic Applications

      • Neil D. Butler, Aditya M. Kunjapur
      Pages 35-53

    5. An Approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions

      • Julianne Peabody, Susan B. Core, Larance Ronsard, Daniel Lingwood, David S. Peabody, Bryce Chackerian
      Pages 55-74

    6. Split-Protein Therapeutic Platforms: Identifying Binder Pairs

      • Christine S. Nervig, James R. Gustat, Shawn C. Owen
      Pages 75-84

    7. Metal-Mediated Ligand Affinity Chemistry (MLAC)

      • Sailajah Gukathasan, Samuel G. Awuah
      Pages 85-97

  3. Engineering Proteins for Therapeutic and Vaccine Applications

    1. Front Matter

      Pages 99-99
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    2. Recombinant Elastin-Based Bioelastomers for Biomedical Applications

      • Paulina M. Babiak, Jason Minnich II, Jessica E. Torres, Sathvik Madduri, Julie C. Liu
      Pages 101-113

    3. Engineering Hepatitis B Virus (HBV) Protein Particles for Therapeutic Delivery

      • Millicent O. Sullivan, Wilfred Chen
      Pages 115-126

    4. Design and Purification of Tag/Catcher AP205-Based Capsid Virus-Like Particle Vaccines

      • Kara-Lee Aves, Adam F. Sander
      Pages 127-141

    5. Protein Nanocarriers Capable of Encapsulating Both Hydrophobic and Hydrophilic Drugs

      • Jooyong Shin, Blair D. Cole, Moosavizadeh Seyedmohammad, Sung In Lim, Yeongseon Jang
      Pages 143-150

    6. Unnatural Amino Acid Engineering for Intracellular Delivery of Protein Therapeutics

      • Wilfred Chen, Millicent O. Sullivan
      Pages 151-164

    7. Formulation of Chitosan–Zein Nano-in-Microparticles for Oral DNA Delivery

      • Kari Heck, Eric Farris, Angela K. Pannier
      Pages 165-176

    8. Engineering E2 Bionanoparticles for Targeted Delivery of Chemotherapeutics to Breast Cancer Cells

      • Millicent O. Sullivan, Wilfred Chen
      Pages 177-189

    9. Constructing Nucleic Acid Delivering Lipoproteoplexes from Coiled-Coil Supercharged Protein and Cationic Liposomes

      • Joseph Thomas, Jonathan Sun, Jin Kim Montclare
      Pages 191-207

    10. Cancer Cell Silicification and Surface Functionalization to Create Microbial Mimetic Cancer Vaccines

      • Lorél Medina, Bryan Kaehr, Rita E. Serda
      Pages 209-219

    11. Melt Processing Virus-Like Particle-Based Vaccine Candidates into Biodegradable Polymer Implants

      • Armando A. Puente, Oscar A. Ortega-Rivera, David M. Wirth, Jonathan K. Pokorski, Nicole F. Steinmetz
      Pages 221-245

  4. Back Matter

    Pages 247-248
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Keywords

About this book

This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and practical, Therapeutic Proteins: Methods and Protocols is a valuable resource for any researcher who are interested in learning more about the field of therapeutic proteins. 


Editors and Affiliations

  • Department of Chemical & Biomolecular Engineering, University of Delaware, Newark, USA

    Millicent O. Sullivan, Wilfred Chen

  • Department of Molecular Genetics and Microbiology School of Medicine, University of New Mexico, Albuquerque, USA

    Bryce Chackerian

Bibliographic Information

  • Book Title: Therapeutic Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Millicent O. Sullivan, Bryce Chackerian, Wilfred Chen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3469-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3468-4Published: 02 October 2023

  • Softcover ISBN: 978-1-0716-3471-4Due: 31 October 2023

  • eBook ISBN: 978-1-0716-3469-1Published: 29 September 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 248

  • Number of Illustrations: 15 b/w illustrations, 47 illustrations in colour

  • Topics: Chemistry/Food Science, general, Behavioral Therapy, Biomedical Engineering and Bioengineering

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