Abstract
Gene gun DiOlistic labelling enables the detailed visualization of retinal ganglion cells (RGCs) dendritic structure. Since the level of labelling is independent of cellular health, it is useful for the characterization of neuronal structure in degenerating neurons where expressed reporters may be inadequate. The method uses compressed helium gas to fire tungsten or gold microparticles coated in carbocyanine dyes (DiD, DiI, DiO) into flat mounted retinas. Here we describe the methods to optimize labelling and ensure a high yield of adequately labelled cells, with a focus on retinal ganglion cells.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Cimaglia, G., Bevan, R.J., Want, A., Morgan, J.E. (2023). Gene Gun DiOlistic Labelling of Retinal Ganglion Cells. In: Mead, B. (eds) Retinal Ganglion Cells. Methods in Molecular Biology, vol 2708. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3409-7_4
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DOI: https://doi.org/10.1007/978-1-0716-3409-7_4
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3408-0
Online ISBN: 978-1-0716-3409-7
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