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Preparation of Retinal Explant Cultures

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Retinal Ganglion Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2708))

Abstract

Organotypic retinal explants are routinely used as alternatives to in vitro cell culture and to replace the use of animals in modelling retinal neurodegenerative diseases. Retinal explants fill the gap between in vivo which are expensive, time consuming, and complex due to inaccessibility of target tissues. However, organotypic retinal explants are less expensive and rapid and retinal cell types in the explant maintain their morphologic interactions with other cells in the retina. Therefore, retinal explants have high potential to be used as tools to assess the pharmacological and other therapies prior to in vivo validation, reducing the use of live animals.

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References

  1. Kolb H, Fernandez E, Nelson R (eds) (1995) The organization of the retina and visual system. University of Utah Health Sciences Center, Salt Lake City

    Google Scholar 

  2. Kim US, Mahroo OA, Mollon JD et al (2021) Retinal ganglion cells – diversity of cell types and clinical relevance. Exp Neurol 12:661938

    Google Scholar 

  3. Sanes JR, Masland RH (2015) The types of retinal ganglion cells: current status and implications for neuronal classification. Annu Rev Neurosci 38:221–246

    Article  CAS  PubMed  Google Scholar 

  4. Dacey DM (2004) Origins of perception: retinal ganglion cell diversity and the creation of parallel visual pathways. In: Gazzaniga MS (ed) The cognitive neurosciences. MIT, Cambridge, MA, pp 281–301

    Google Scholar 

  5. Goldberg JL, Corredor RG (2009) Retinal ganglion cell life and death – mechanisms and implications for ophthalmology. Eur Ophthalmol Rev 2:109–112

    Google Scholar 

  6. Yamada ES, Bordt AS, Marshak DW (2005) Wide-field ganglion cells in macaque retinas. Vis Neurosci 22:383–393

    Article  PubMed  PubMed Central  Google Scholar 

  7. Masri RA, Percival KA, Koizumi A et al (2019) Survey of retinal ganglion cell morphology in marmoset. J Comp Neurol 527:236–558

    Article  CAS  PubMed  Google Scholar 

  8. Diamond JS (2017) Inhibitory interneurons in the retina: types, circuitry, and function. Annu Rev Vis Sci 3:1–24

    Article  PubMed  Google Scholar 

  9. Ahmed Z, Suggate EL, Brown ER et al (2006) Schwann cell-derived factor-induced modulation of the NgR/p75NTR/EGFR axis disinhibits axon growth through CNS myelin in vivo and in vitro. Brain 129:1517–1533

    Article  PubMed  Google Scholar 

  10. Vigneswara V, Akpan N, Berry M et al (2014) Combined suppression of CASP2 and CASP6 protects retinal ganglion cells from apoptosis and promotes axon regeneration through CNTF-mediated JAK/STAT signalling. Brain 137:1656–1675

    Article  PubMed  PubMed Central  Google Scholar 

  11. Ahmed Z, Kalinski H, Berry M et al (2011) Ocular neuroprotection by siRNA targeting caspase-2. Cell Death Dis 2:e173

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Busskamp V, Duebel J, Balya D et al (2010) Genetic reactivation of cone photoreceptors restores visual responses in retinitis pigmentosa. Science 329:413–417

    Article  CAS  PubMed  Google Scholar 

  13. Valdés J, Trachsel-Moncho L, Sahaboglu A et al (2016) Organotypic retinal explant cultures as in vitro alternative for diabetic retinopathy studies. ALTEX 33:459–464

    PubMed  Google Scholar 

  14. Caffé AR, Ahuja P, Holmqvist B et al (2001) Mouse retina explants after long-term culture in serum free medium. J Chem Neuroanat 22:263–273

    Article  PubMed  Google Scholar 

  15. Xin H, Yannazzo J, Duncan R et al (2007) A novel organotypic culture model of the postnatal mouse retina allows the study of glutamate-mediated excitotoxicity. J Neurosci Methods 159:35–42

    Article  CAS  PubMed  Google Scholar 

  16. Johnson T, DeKorver N, Levasseur V et al (2014) Identification of retinal ganglion cell neuroprotection conferred by platelet-derived growth factor through analysis of the mesenchymal stem cell secretome. Brain 137:503–519

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK

    Zubair Ahmed

Authors
  1. Zubair Ahmed
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Corresponding author

Correspondence to Zubair Ahmed .

Editor information

Editors and Affiliations

  1. School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK

    Ben Mead

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Ahmed, Z. (2023). Preparation of Retinal Explant Cultures. In: Mead, B. (eds) Retinal Ganglion Cells. Methods in Molecular Biology, vol 2708. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3409-7_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3409-7_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3408-0

  • Online ISBN: 978-1-0716-3409-7

  • eBook Packages: Springer Protocols

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