Abstract
Optical coherence tomography (OCT) enables micron-scale resolution of structural anatomy, thereby making OCT a valuable tool for addressing ophthalmologic and neurologic inquiries. Although the murine eye and its structures are very small and offers challenges for OCT imaging, OCT can be used to monitor retinal layer thickness in healthy and diseased retinas in murine lines in vivo longitudinally. Thus, OCT can provide insights into disease severity and treatment efficacy. This chapter describes the use of OCT as a powerful non-invasive imaging technology for high-resolution retinal imaging and retinal thickness quantification in rodents.
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Acknowledgments
The Bascom Palmer Eye Institute was supported by NIH Center Core Grant P30EY014801 and a Research to Prevent Blindness Unrestricted Grant. R.K. Lee is partially supported by the Walter G. Ross Foundation. This work was partly supported by the Gutierrez Family Research Fund, the Camiener Family Glaucoma Research Fund, and the National Natural Science Foundation of China (No. 82201170 to X.L.).
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Liu, X., Liu, Y., Lee, R.K. (2023). Optical Coherence Tomography: Imaging Visual System Structures in Mice. In: Mead, B. (eds) Retinal Ganglion Cells. Methods in Molecular Biology, vol 2708. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3409-7_11
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DOI: https://doi.org/10.1007/978-1-0716-3409-7_11
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