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Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish

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Zebrafish

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2707))

Abstract

Understanding how motor circuits are organized and recruited in order to perform complex behavior is an essential question of neuroscience. Here we present an optogenetic protocol on larval zebrafish that allows spatial selective control of neuronal activity within a genetically defined population. We combine holographic illumination with the use of effective opsin transgenic lines, alongside high-speed behavioral monitoring to dissect the motor circuits of the larval zebrafish.

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Acknowledgments

The authors would like to acknowledge Martin Carbó-Tano for the development of the behavioral monitoring protocol including the 3D printed claw and the enucleation protocol that leads to Fig. 2. in this chapter and Mathilde Lapoix for her advice in the implementation of the behaviour protocol and the members of the Wyart Lab for feedback and discussion. We also thank Dimitrii Tanese, Nelson Rebola and Joana Guedes for proofreading of the manuscript. We thank Ben Shababo and Osnath Assayag from Intelligent Imaging for the technical support on using Phasor. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement #813457 as well as Fondation pour la Recherche Médicale (FRM, grant #EQU202003010612) and the Fondation Bettencourt-Schueller (FBS, grant #Don0063).

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Authors and Affiliations

  1. Sorbonne Université, Institut du Cerveau (ICM), Paris, France

    Xinyu Jia & Claire Wyart

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  1. Xinyu Jia
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  2. Claire Wyart
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Correspondence to Claire Wyart .

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Editors and Affiliations

  1. Department of Pediatrics Children’s Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    James F. Amatruda

  2. Centre for Developmental Neurobiology, MRC Centre for NeuroDevelopmental Disorders, King’s College London, London, UK

    Corinne Houart

  3. Laboratory of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan

    Koichi Kawakami

  4. Duke Regeneration Center, Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

    Kenneth D. Poss

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Jia, X., Wyart, C. (2024). Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish. In: Amatruda, J.F., Houart, C., Kawakami, K., Poss, K.D. (eds) Zebrafish. Methods in Molecular Biology, vol 2707. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3401-1_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3401-1_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3400-4

  • Online ISBN: 978-1-0716-3401-1

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