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SH2 Domains

Functional Modules and Evolving Tools in Biology

  • Book
  • © 2023

Overview

Editors:
  1. Teresa Carlomagno

    1. School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Maja Köhn

    1. Signalling Research Centres BIOSS &CIBSS, University of Freiburg, Freiburg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2705)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Structure and Dynamics of SH2 Domains

    1. Front Matter

      Pages 1-1
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    2. Methods for Structure Determination of SH2 Domain–Phosphopeptide Complexes by NMR

      • Vittoria Nanna, Michelangelo Marasco, John P. Kirkpatrick, Teresa Carlomagno
      Pages 3-23

    3. NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes

      • Michelangelo Marasco, John P. Kirkpatrick, Vittoria Nanna, Teresa Carlomagno
      Pages 25-37

    4. Crystal Structure Analysis of SH2 Domains in Complex with Phosphotyrosine Peptides

      • Miki Senda, Toshiya Senda
      Pages 39-58

    5. Revealing Allostery in PTPN11 SH2 Domains from MD Simulations

      • Massimiliano Anselmi, Jochen S. Hub
      Pages 59-75

    6. Structure Determination of SH2–Phosphopeptide Complexes by X-Ray Crystallography: The Example of p120RasGAP

      • Amy L. Stiegler, Titus J. Boggon
      Pages 77-89

  3. Biophysics and Bioinformatics of SH2 Domain–Phosphopeptide Binding

    1. Front Matter

      Pages 91-91
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    2. Fluorescence Anisotropy and Polarization in the Characterization of Biomolecular Association Processes and Their Application to Study SH2 Domain Binding Affinity

      • Sara Bobone, Claudia Storti, Paolo Calligari, Lorenzo Stella
      Pages 93-112

    3. Computational Evaluation of Peptide–Protein Binding Affinities: Application of Potential of Mean Force Calculations to SH2 Domains

      • Paolo Calligari, Lorenzo Stella, Gianfranco Bocchinfuso
      Pages 113-133

    4. NMR Relaxation Dispersion Experiments to Study Phosphopeptide Recognition by SH2 Domains: The Grb2-SH2–Phosphopeptide Encounter Complex

      • Fabio C. L. Almeida, Karoline Sanches, Icaro P. Caruso, Fernando A. Melo
      Pages 135-151

    5. Using Linear Motif Database Resources to Identify SH2 Domain Binders

      • Hugo Sámano-Sánchez, Toby J. Gibson, Lucía B. Chemes
      Pages 153-197

    6. Using Surface Plasmon Resonance to Study SH2 Domain–Peptide Interactions

      • Gabrielle M. Watson, Menachem J. Gunzburg, Jacqueline A. Wilce
      Pages 199-210

  4. Small-Molecule Binders and Inhibitors of SH2 Domains

    1. Front Matter

      Pages 211-211
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    2. Inhibitor Library Screening of SH2 Domains Through Denaturation-Based Assays

      • Elvin D. de Araujo, Anna Orlova, Qirat F. Ashraf, Richard Moriggl, Patrick T. Gunning
      Pages 213-223

    3. Dissecting Selectivity Determinants of Small-Molecule Inhibitors of SH2 Domains Via Fluorescence Polarization Assays

      • Angela Berg, Julian Gräb, Barbara Klüver, Thorsten Berg
      Pages 225-238

    4. Lipid Binding of SH2 Domains

      • Wonhwa Cho, Kyli Berkley, Ashutosh Sharma
      Pages 239-253

    5. Exploring the Binding Interaction Between Phosphotyrosine Peptides and SH2 Domains by Proximal Crosslinking

      • Rui Wang, Yishu Bao, Jiang Xia
      Pages 255-267

    6. Synthesis and Biochemical Evaluation of Monocarboxylic GRB2 SH2 Domain Inhibitors

      • Tao Xiao, Min Zhang, Haitao Ji
      Pages 269-290

  5. Engineering SH2 Domains

    1. Front Matter

      Pages 291-291
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Keywords

About this book

This volume looks at the latest methods used to study and modulate the biological function and mechanisms of SH2 domains. The chapters in this book are organized into five parts. Part One presents methodology aimed at determining the structures and dynamics of SH2 domains and their complexes with phosphopeptides. Part Two discusses techniques to understand and predict interactions of SH2 domains by measuring or calculating their affinity to phosphopeptides. Part Three focuses on inhibitors of SH2 domains that lead the way for chemical tool development and drug discovery. Part Four describes how to evolve and engineer SH2 domains with specific binding properties, and Part Five explores how to measure the regulation of protein tyrosine phosphatase activity through allosteric binding of peptides to SH2 domains and condensation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, SH2 Domains: Functional Modules and Evolving Tools in Biology is a valuable resource for researchers, working in the biophysical and biochemical field, who want to learn more about this exciting and versatile class of regulatory and signaling domains. 


Editors and Affiliations

  • School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, UK

    Teresa Carlomagno

  • Signalling Research Centres BIOSS &CIBSS, University of Freiburg, Freiburg, Germany

    Maja Köhn

Bibliographic Information

  • Book Title: SH2 Domains

  • Book Subtitle: Functional Modules and Evolving Tools in Biology

  • Editors: Teresa Carlomagno, Maja Köhn

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3393-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3392-2Published: 06 September 2023

  • Softcover ISBN: 978-1-0716-3395-3Due: 07 October 2023

  • eBook ISBN: 978-1-0716-3393-9Published: 05 September 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 382

  • Number of Illustrations: 10 b/w illustrations, 57 illustrations in colour

  • Topics: Cell Biology, Life Sciences, general, Chemistry/Food Science, general

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