Abstract
Penicillin-binding protein-type thioesterases (PBP-type TEs) are an emerging family of non-ribosomal peptide cyclases. PBP-type TEs exhibit distinct substrate scopes from the well-exploited ribosomal peptide cyclases and traditional non-ribosomal peptide cyclases. Their unique properties, as well as their stand-alone nature, highlight PBP-type TEs as valuable candidates for development as biocatalysts for peptide macrocyclization. Here in this chapter, we describe the scheme for the chemoenzymatic synthesis of non-ribosomal macrolactam by SurE, a representative member of PBP-type TEs.
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Acknowledgement
This work was partly supported by Hokkaido University, Global Facility Center (GFC), Pharma Science Open Unit (PSOU), funded by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) under “Support Program for Implementation of New Equipment Sharing System”, Global Station for Biosurfaces and Drug Discovery, a project of Global Institution for Collaborative Research and Education in Hokkaido University, the Asahi Glass Foundation, the Naito Foundation, the Uehara Memorial Foundation, the Sumitomo Foundation−Grant for Basic Science Research Projects, Daiichi Sankyo Foundation of Life Science, Japan Foundation for Applied Enzymology, TERUMO Life Science Foundation, the Japan Agency for Medical Research and Development JP19ae0101045, the Japan Science and Technology Agency (JST Grant Numbers ACT-X JPMJAX201F and A-STEP JPMJTR20US), Japan JP16H06448, JP18H02581, JP19K16390, JP21H02635, JP22K15302 and JP22J13818.
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Kobayashi, M., Fujita, K., Matsuda, K., Wakimoto, T. (2023). Chemo-Enzymatic Synthesis of Non-ribosomal Macrolactams by a Penicillin-Binding Protein-Type Thioesterase. In: Burkart, M., Ishikawa, F. (eds) Non-Ribosomal Peptide Biosynthesis and Engineering. Methods in Molecular Biology, vol 2670. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3214-7_6
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DOI: https://doi.org/10.1007/978-1-0716-3214-7_6
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