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Genetic Mouse Models of Pneumocystis Pneumonia

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Antifungal Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2667))

Abstract

Pneumocystis jirovecii causes pneumonia in immunocompromised patients. A major challenge in drug susceptibility testing and in understanding host/pathogen interactions is that Pneumocystis spp. are not viable in vitro. Continuous culture of the organism is not currently available, and therefore, developing new drug targets is very limited. Due to this limitation, mouse models of Pneumocystis pneumonia have proven to be an invaluable resource to researchers. In this chapter, we provide an overview of selected methods used in mouse models of infection including, in vivo Pneumocystis murina propagation, routes of transmission, genetic mouse models available, a P. murina life form-specific model, a mouse model of PCP immune reconstitution inflammatory syndrome (IRIS), and the experimental parameters associated with these models.

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Author information

Authors and Affiliations

  1. AFRICA CMM Medical Mycology Research Unit, Institute of Infectious Disease and Molecular Medicine (IDM), Cape Town, South Africa

    J. Claire Hoving

  2. Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa

    J. Claire Hoving

  3. MRC Centre for Medical Mycology, University of Exeter, Exeter, UK

    J. Claire Hoving

  4. Centre for Translational Research in Infection and Inflammation, Tulane School of Medicine, New Orleans, LA, USA

    Ferris T. Munyonho & Jay K. Kolls

Authors
  1. J. Claire Hoving
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  2. Ferris T. Munyonho
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  3. Jay K. Kolls
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Corresponding authors

Correspondence to J. Claire Hoving or Jay K. Kolls .

Editor information

Editors and Affiliations

  1. Institute of Immunology & Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK

    Rebecca A. Drummond

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Hoving, J.C., Munyonho, F.T., Kolls, J.K. (2023). Genetic Mouse Models of Pneumocystis Pneumonia. In: Drummond, R.A. (eds) Antifungal Immunity. Methods in Molecular Biology, vol 2667. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3199-7_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3199-7_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3198-0

  • Online ISBN: 978-1-0716-3199-7

  • eBook Packages: Springer Protocols

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