Abstract
Bacteria sense and respond to gaseous ligand changes in the environment to regulate a multitude of behaviors, including the production of the secondary messengers cyclic di-GMP. Gas sensing can be difficult to measure due to the high concentration of the oxygen in the atmosphere, particularly in redox-sensitive systems. Here, we describe a method for anaerobic quantification of cyclic di-GMP production which can be used to measure the impact of molecular oxygen, nitric oxide, and carbon monoxide on the catalysis of a diguanylate cyclase-containing protein and the possible pitfalls in the experimental procedure.
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Acknowledgements
This work was supported by NSF grants CHE1352040 (E.E.W.) and CHE2003350 (E.E.W.) and Frasch Foundation grant 824-H17 (E.E.W.).
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Hoque, N.J., Helm, M.P., Weinert, E.E. (2023). In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity. In: Weinert, E.E. (eds) Oxygen Sensing. Methods in Molecular Biology, vol 2648. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3080-8_6
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DOI: https://doi.org/10.1007/978-1-0716-3080-8_6
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