Abstract
Certain viral pathogens can be shed into the human breast milk and cause infections in the infant upon breastfeeding. Thus, it is important to clarify whether viral RNA as well as infectious virus can be found in breast milk. The complexity of this body fluid poses several challenges for viral RNA isolation and detection of infectious virus. We here provide a protocol that allowed the identification of SARS-CoV-2 RNA in breast milk and the isolation of infectious virus after the virus has been artificially spiked into milk samples.
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Acknowledgments
CC and RG are part of the International Graduate School in Molecular Medicine Ulm. JAM received funding from the German Research Foundation.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Groß, R., Conzelmann, C., Münch, J., Müller, J.A. (2023). Detection of SARS-CoV-2 in Human Breast Milk. In: Aquino de Muro, M. (eds) Virus-Host Interactions. Methods in Molecular Biology, vol 2610. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2895-9_11
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DOI: https://doi.org/10.1007/978-1-0716-2895-9_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2894-2
Online ISBN: 978-1-0716-2895-9
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