Abstract
Digenome-seq is a powerful approach for determining the genome-wide specificity of programmable nuclease including CRISPR-Cas9 and CRISPR-Cpf1 (also known as Cas12a) and programmable deaminase including cytosine base editors (CBEs) and adenine base editors (ABEs). To define the genome-wide specificity of dLbCpf1-BE (also known as dLbCas12a-BE), genomic DNA is first incubated with dLbCpf1-BE, which induces C-to-U conversion at on-target and off-target sites, and then treated with a mixture of E. coli uracil DNA glycosylase (UDG) and Endonuclease VIII, which creates single-strand breaks (SSBs) by removing uracil in vitro. Digested genomic DNA is subjected to WGS, and then sequencing reads are aligned to the reference genome, resulting in straight alignments at on-target and off-target sites. The in vitro cleavage sites related to the straight alignments can be identified using the Digenome-seq computer tool.
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References
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Kim, D. (2023). Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq. In: Bae, S., Song, B. (eds) Base Editors. Methods in Molecular Biology, vol 2606. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2879-9_4
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DOI: https://doi.org/10.1007/978-1-0716-2879-9_4
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