Abstract
Leaf epidermis pavement cells form highly complex shapes with interlocking lobes and necks at their anticlinal walls. The microtubule cytoskeleton plays essential roles in pavement cell morphogenesis, in particular at necks. Vice versa, shape generates stress patterns that regulate microtubule organization. Genetic or pharmacological perturbations that affect pavement cell shape often affect microtubule organization. Pavement cell shape and microtubule organization are therefore closely interconnected. Here, we present commonly used approaches for the quantitative analysis of pavement cell shape characteristics and of microtubule organization. In combination with ablation experiments, these methods can be applied to investigate how different genotypes (or treatments) affect the organization and stress responsiveness of the microtubule cytoskeleton.
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Acknowledgments
Katharina Bürstenbinder would like to thank the Deutsche Forschungsgemeinschaft (DFG) (grant numbers BU2955/2-1 and BU2955/1-2), the German-Israeli Foundation for Scientific Research and Development (GIF) (grant number G-1482-423.13/2018), and the Leibniz Association for funding.
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Klemm, S., Buhl, J., Möller, B., Bürstenbinder, K. (2023). Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells. In: Hussey, P.J., Wang, P. (eds) The Plant Cytoskeleton. Methods in Molecular Biology, vol 2604. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2867-6_4
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DOI: https://doi.org/10.1007/978-1-0716-2867-6_4
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